MPV17 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 4.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 4
Alternative names
Glomerulosclerosis, MPV17_HUMAN, MTDPS6, MpV17 mitochondrial inner membrane protein, Mpv17 human homolog of glomerulosclerosis and nephrotic syndrome, Protein Mpv17, SYM1
Recommended products
MPV17 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 4.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 4
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- MPV17
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MPV17 also known as MPV17 mitochondrial inner membrane protein plays an important role in the integrity of mitochondrial DNA (mtDNA) maintenance. It is a 20 kDa protein found primarily in the inner mitochondrial membrane. Expression of MPV17 occurs in various tissues notably in the liver kidney and central nervous system. The protein localizes to mitochondria where it is involved in maintaining mitochondrial function.
- Biological function summary
MPV17 affects several processes related to cellular energy production. It is part of a larger protein family involved in mitochondrial homeostasis. MPV17 contributes to the regulation of oxidative phosphorylation by modulating mitochondrial DNA content. This regulation assures proper function of the respiratory chain complexes required for cellular energy provision. Additionally its activity helps prevent mtDNA damage that may arise from oxidative stress.
- Pathways
MPV17 influences energy metabolism pathways critical for cell viability. It interacts with the mitochondrial DNA maintenance pathway facilitating the replication and distribution of mtDNA within cells. MPV17 has functional associations with other proteins like POLG which is the catalytic subunit of the mitochondrial DNA polymerase responsible for replication and repair. This relationship ensures the stability and transcription of mitochondrial genome necessary for proper cellular energy management.
- Associated diseases and disorders
Mutations in MPV17 lead to conditions like mitochondrial DNA depletion syndrome (MDDS) and Navajo neurohepatopathy. MDDS results in reduced mtDNA levels affecting energy production and leading to severe liver and neurological disorders. The disease links MPV17 to other proteins involved in mitochondrial maintenance such as TWNK another protein necessary for mtDNA maintenance and expression. Understanding MPV17’s role in these pathologies can provide insights into potential therapeutic strategies for corresponding mitochondrial disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sanger Sequencing - Human MPV17 knockout HeLa cell line (ab265083)
Homozygous: 1 bp deletion in exon 4.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com