MSX1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1
Alternative names
AA675338, AI324650, HYD 1, Homeobox 7, Homeobox protein Hox-7, Homeobox protein MSX-1, Homeobox, msh-like 1, Hox 7.1, Hox-7, MSH, MSH, Drosophila, Homolog of, 1, MSX1_HUMAN, Msh homeobox 1, Msh homeobox 1-like protein, Muscle segment homeobox, Muscle segment homeobox, Drosophila, Homolog of, 1, OFC5, OTTHUMP00000115387, STHAG1, msh (Drosophila) homeo box homolog 1 (formerly homeo box 7), msh homeo box homolog 1, msh homeobox homolog 1 (Drosophila)
Recommended products
MSX1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- MSX1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
- Culture medium
- F-12K + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MSX1 protein sometimes called Homeobox protein MSX-1 is a transcription factor with a mass of approximately 33 kDa. It plays a role in the regulation of embryonic development by controlling the expression of several genes. This protein is mainly expressed in tissues such as the limb buds craniofacial regions and developing teeth which are critical sites for its function during different stages of growth.
- Biological function summary
MSX1 modulates a variety of developmental processes including craniofacial morphogenesis and limb formation. It operates as part of a DNA-binding complex that influences the transcription of target genes essential for proper tissue differentiation and formation. Through its activity MSX1 affects cell proliferation and apoptosis ensuring the accurate development of specific structures in the organism.
- Pathways
MSX1 interacts with the WNT signaling and bone morphogenetic protein (BMP) pathways. In the WNT signaling pathway MSX1 modulates the expression of genes involved in the regulation of developmental processes and cell fate determination. It also interacts with BMP-related proteins playing an important role in skeletal formation and patterning demonstrating connections with other proteins like BMP4 and LEF1.
- Associated diseases and disorders
MSX1 has been implicated in the development of tooth agenesis and craniosynostosis. Research shows that mutations in the MSX1 gene can lead to these conditions affecting the normal formation and differentiation of skeletal elements. Furthermore MSX1 has connections with the protein PAX9 as together they contribute to the regulation of dental development and disruptions in their interactions often result in anomalies in tooth formation.
Product promise
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1 product image
Sanger Sequencing - Human MSX1 knockout A549 cell line (ab267157)
Homozygous: 1 bp deletion in exon1
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