MTA1 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9 X = 5 bp deletion after Arg24 of the WT protein
Frameshift: 100%.
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Key facts
- Cell type
- HEK-293
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 5 bp deletion after Arg24 of the WT protein Frameshift: 100%
Alternative names
MTA1_HUMAN, Metastasis associated 1, Metastasis associated gene 1, Metastasis associated gene 1 protein, Metastasis associated protein, Metastasis associated protein MTA 1, Metastasis-associated protein MTA1
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MTA1 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9 X = 5 bp deletion after Arg24 of the WT protein
Frameshift: 100%.
Key facts
- Cell type
- HEK-293
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 5 bp deletion after Arg24 of the WT protein Frameshift: 100%
- Concentration
Properties
- Gene name
- MTA1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MTA1 protein short for Metastasis-Associated 1 is a significant player in cellular mechanisms. It has an approximate molecular weight of 80 kDa. MTA1 is known for its function as a chromatin remodeling factor involved in altering chromatin structure to regulate gene expression. Alternate names include metastasis tumor antigen 1. MTA1 expresses widely but has higher levels in metastatic tumor cells playing a role in cancer progression by influencing gene transcription and chromatin dynamics.
- Biological function summary
MTA1 contributes to regulating diverse cellular processes. It forms part of the NuRD complex which plays a critical role in mediating chromatin remodeling and histone deacetylation. This association enables MTA1 to influence key aspects of cellular behavior including cell proliferation differentiation and response to stress. The protein's involvement extends to modulating transcription factors and interacting with several other proteins impacting cellular growth and metastasis.
- Pathways
MTA1 participates actively in the regulation of several key signaling networks. One significant pathway is the Wnt/β-catenin signaling pathway where MTA1 modulates gene expression by interacting with β-catenin. In another pathway it affects the estrogen receptor signaling pathway impacting gene transcription in hormone-dependent cancers. MTA1’s interaction with HDAC (Histone deacetylase) proteins emphasizes its role in epigenetic regulation across different biological pathways.
- Associated diseases and disorders
MTA1 is frequently connected with cancer progression and metastasis. Its overexpression correlates with poor prognosis in breast cancer highlighting its impact on tumor aggressiveness. Additionally MTA1 has implications in prostate cancer where it interacts with the androgen receptor influencing cancer cell proliferation and resistance to therapy. Understanding the role of MTA1 in these diseases could potentially lead to new therapeutic targets for cancer treatment.
Product promise
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1 product image
Next Generation Sequencing - Human MTA1 knockout HEK-293 cell line (ab277164)
5 bp deletion after Arg24 of the WT protein
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