MTHFD1L KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 8 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 8 bp deletion in exon 2 and Insertion of the selection cassette in exon 2
Alternative names
10-formyl-THF synthetase, C1TM_HUMAN, FTHFSDC1, Formyltetrahydrofolate synthetase, MTC1THFS, Monofunctional C1-tetrahydrofolate synthase, RP1-292B18.2, formyltetrahydrofolate synthetase domain containing 1, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like, mitochondrial
Recommended products
MTHFD1L KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 8 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 8 bp deletion in exon 2 and Insertion of the selection cassette in exon 2
- Concentration
Properties
- Gene name
- MTHFD1L
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MTHFD1L also known as methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like functions as an enzyme participating in folate metabolism. With an approximate mass of 105 kDa MTHFD1L is active in the mitochondria. This enzyme facilitates the interconversion of tetrahydrofolate derivatives supporting the folate-mediated one-carbon metabolic pathway. MTHFD1L shows expression in various tissues with a noticeable presence in liver and embryonic tissues where it has roles in embryogenesis and tissue-specific functions.
- Biological function summary
MTHFD1L catalyzes reactions converting methylene-THF to methenyl-THF and then to formyl-THF utilizing NADP+ as a cofactor. It does not function as a standalone component; instead it may operate as part of a larger complex engaged in one-carbon metabolism. Its activity contributes to the synthesis of important biomolecules such as nucleotides and amino acids emphasizing its role in DNA synthesis and repair.
- Pathways
The folate-mediated one-carbon metabolism uses MTHFD1L to manage one-carbon units. This enzyme's function fits into the folate pathway important for nucleotide biosynthesis. It connects to other proteins such as MTHFD1 and SHMT1 which also partake in folate metabolism supporting interconnected biochemical pathways in various cellular processes.
- Associated diseases and disorders
Defects or alterations in MTHFD1L are linked to neural tube defects and some cancers. The enzyme's involvement in embryonic development and DNA repair suggests that mutations or dysregulation may contribute to these conditions. MTHFD1 and SHMT1 related proteins have similar associations highlighting their collective impact on disease when there is impaired folate metabolism.
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3 product images
Sanger Sequencing - Human MTHFD1L knockout HEK-293T cell line (ab266135)
Allele-1: 8 bp deletion in exon2
Sanger Sequencing - Human MTHFD1L knockout HEK-293T cell line (ab266135)
Allele-2: 1 bp insertion in exon 2.
Sanger Sequencing - Human MTHFD1L knockout HEK-293T cell line (ab266135)
Allele-3: Insertion of the selection cassette in exon 2.
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