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AB256500

Human MYC (c-Myc) knockout HEK-293T cell line

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(1 Review)

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(1 Publication)

MYC KO cell line available to order. KO validated by Immunohistochemistry, Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous (4N): 1bp T insertion (2N); 8 bp deletion and C to T insertion (1N); 4 bp deletion in exon 2 (1N). To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.

View Alternative Names

AU016757, Avian myelocytomatosis viral oncogene homolog, Cellular myelocytomatosis oncogene, Class E basic helix-loop-helix protein 39, MGC105490, MRTL, MYCC, MYC_HUMAN, Myc protein, Myc proto-oncogene protein, Myc2, Myelocytomatosis oncogene, Myelocytomatosis oncogene a, Niard, Nird, OTTHUMP00000158589, OTTHUMP00000227763, Oncogene Myc, Proto-oncogene c-Myc, Protooncogene homologous to myelocytomatosis virus, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, V-Myc avian myelocytomatosis viral oncogene homolog, bHLHe39, c Myc, myc-related translation/localization regulatory factor, myca, oncogene c-Myc, v-myc myelocytomatosis viral oncogene homolog (avian), zc-myc

14 Images
Immunocytochemistry/ Immunofluorescence - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

ab32072 staining MYC in wild-type HEK293 cells (top panel) and MYC knockout HEK293 cells (ab256500) (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab32072 at 5μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Western blot - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • WB

Lab

Western blot - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

False colour image of Western blot : Anti-c-Myc antibody [Y69] staining at 1/1000 dilution shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution shown in red. In Western blot ab32072 was shown to bind specifically to c-Myc. A band was observed at 45/57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line ab256500 (CRISPR-Cas9 edited cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 45/57 kDa is likely to represent a truncated form of c-Myc. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-c-Myc antibody [Y69] - ChIP Grade (<a href='/en-us/products/primary-antibodies/c-myc-antibody-y69-chip-grade-ab32072'>ab32072</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human MYC (c-Myc) knockout HEK-293T cell lysate (<a href='/en-us/products/cell-lysates/human-myc-c-myc-knockout-hek-293t-cell-lysate-ab263850'>ab263850</a>) at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 48 kDa

Observed band size: 45 kDa,57 kDa

false

Western blot - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • WB

Lab

Western blot - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

False colour image of Western blot : Anti-Myc tag antibody [9E10] staining at 1/200 dilution shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution shown in red. In Western blot ab32 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line ab256500 (CRISPR-Cas9 edited cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

Lanes 1 - 4:

Western blot - Anti-Myc tag antibody [9E10] (<a href='/en-us/products/primary-antibodies/myc-tag-antibody-9e10-ab32'>ab32</a>) at 1/200 dilution

Lanes 1 - 4:

Western blot - Anti-Myc tag antibody [9E10] (<a href='/en-us/products/primary-antibodies/myc-tag-antibody-9e10-ab206486'>ab206486</a>) at 1/200 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

MYC CRISPR-Cas9 edited HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human MYC (c-Myc) knockout HEK-293T cell line (ab256500)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 48 kDa,49 kDa

false

Western blot - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • WB

Lab

Western blot - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

False colour image of Western blot : Anti-Myc tag antibody [Myc.A7] staining at 1/1000 dilution shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution shown in red. In Western blot ab18185 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line ab256500 (CRISPR-Cas9 edited cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-Myc tag antibody [Myc.A7] (<a href='/en-us/products/primary-antibodies/myc-tag-antibody-myca7-ab18185'>ab18185</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

MYC CRISPR-Cas9 edited HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human MYC (c-Myc) knockout HEK-293T cell line (ab256500)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 48 kDa,49 kDa

false

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

CUT&RUN profiling with c-Myc antibody reveals the expected genomic enrichment pattern in wild-type (WT) cells, which is substantially reduced in MYC knockout (KO) cells. Representative genome browser tracks show CUT&RUN data generated using the CUTANA™ CUT&RUN Kit (EpiCypher 14-1048) with c-Myc antibody (Abcam ab32072, 0.5 µg). 500,000 HEK293T WT or KO (Abcam ab256500) cells were used per reaction. IgG, H3K4me3, and H3K27me3 antibodies were included as controls to assess non-specific background, active promoters, and repressed chromatin, respectively. Libraries were prepared using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001). Sequencing was performed with paired-end 50 bp reads, and data were processed on CUTANA™ Cloud (cloud.epicypher.com) by alignment to the hg38 genome. Images were generated using Integrative Genomics Viewer (IGV, Broad Institute).

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

CUT&RUN profiling with c-Myc antibody reveals the expected genomic enrichment pattern in wild-type (WT) cells, which is substantially reduced in MYC knockout (KO) cells. Representative genome browser tracks show CUT&RUN data generated using the CUTANA™ CUT&RUN Kit (EpiCypher 14-1048) with c-Myc antibody (Abcam ab290675, 0.5 µg). 500,000 HEK293T WT or KO (Abcam ab256500) cells were used per reaction. IgG, H3K4me3, and H3K27me3 antibodies were included as controls to assess non-specific background, active promoters, and repressed chromatin, respectively. Libraries were prepared using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001). Sequencing was performed with paired-end 50 bp reads, and data were processed on CUTANA™ Cloud (cloud.epicypher.com) by alignment to the hg38 genome. Images were generated using Integrative Genomics Viewer (IGV, Broad Institute).

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

CUT&RUN profiling with c-Myc antibody demonstrates robust genome-wide enrichment in wild-type (WT) cells, which is markedly diminished in MYC knockout (KO) cells. Heatmaps of genome-wide signal flanking annotated transcription start sites (TSSs, +/- 2 kbp) display CUT&RUN data generated using the CUTANA™ CUT&RUN Kit (EpiCypher 14-1048) with c-Myc antibody (Abcam ab290674, 0.5 µg). 500,000 HEK293T WT or KO (Abcam ab256500) cells were used per reaction. IgG antibody was included as a negative control to assess non-specific background. Libraries were prepared using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001). Sequencing was performed with paired-end 50 bp reads, and data were processed on CUTANA™ Cloud (cloud.epicypher.com) by alignment to the hg38 genome. Heatmaps were generated using deepTools (Ramнrez et al., Nucleic Acids Res. 2014; PMID : 24799436). Row-linked data are ranked by intensity relative to c-Myc WT, with red indicating high localized enrichment and blue denoting background. Validated antibodies show genome-wide enrichment above IgG background consistent with c-Myc binding in WT cells and near complete loss of signal in KO cells.

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

CUT&RUN profiling with c-Myc antibody demonstrates robust genome-wide enrichment in wild-type (WT) cells, which is markedly diminished in MYC knockout (KO) cells. Heatmaps of genome-wide signal flanking annotated transcription start sites (TSSs, +/- 2 kbp) display CUT&RUN data generated using the CUTANA™ CUT&RUN Kit (EpiCypher 14-1048) with c-Myc antibody (Abcam ab185656, 0.5 µg). 500,000 HEK293T WT or KO (Abcam ab256500) cells were used per reaction. IgG antibody was included as a negative control to assess non-specific background. Libraries were prepared using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001). Sequencing was performed with paired-end 50 bp reads, and data were processed on CUTANA™ Cloud (cloud.epicypher.com) by alignment to the hg38 genome. Heatmaps were generated using deepTools (Ramírez et al., Nucleic Acids Res. 2014; PMID : 24799436). Row-linked data are ranked by intensity relative to c-Myc WT, with red indicating high localized enrichment and blue denoting background. Validated antibodies show genome-wide enrichment above IgG background consistent with c-Myc binding in WT cells and near complete loss of signal in KO cells.

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

CUT&RUN profiling with c-Myc antibody demonstrates robust genome-wide enrichment in wild-type (WT) cells, which is markedly diminished in MYC knockout (KO) cells. Heatmaps of genome-wide signal flanking annotated transcription start sites (TSSs, +/- 2 kbp) display CUT&RUN data generated using the CUTANA™ CUT&RUN Kit (EpiCypher 14-1048) with c-Myc antibody (Abcam ab290675, 0.5 µg). 500,000 HEK293T WT or KO (Abcam ab256500) cells were used per reaction. IgG antibody was included as a negative control to assess non-specific background. Libraries were prepared using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001). Sequencing was performed with paired-end 50 bp reads, and data were processed on CUTANA™ Cloud (cloud.epicypher.com) by alignment to the hg38 genome. Heatmaps were generated using deepTools (Ramнrez et al., Nucleic Acids Res. 2014; PMID : 24799436). Row-linked data are ranked by intensity relative to c-Myc WT, with red indicating high localized enrichment and blue denoting background. Validated antibodies show genome-wide enrichment above IgG background consistent with c-Myc binding in WT cells and near complete loss of signal in KO cells.

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

CUT&RUN profiling with c-Myc antibody reveals the expected genomic enrichment pattern in wild-type (WT) cells, which is substantially reduced in MYC knockout (KO) cells. Representative genome browser tracks show CUT&RUN data generated using the CUTANA™ CUT&RUN Kit (EpiCypher 14-1048) with c-Myc antibody (Abcam ab290674, 0.5 µg). 500,000 HEK293T WT or KO (Abcam ab256500) cells were used per reaction. IgG, H3K4me3, and H3K27me3 antibodies were included as controls to assess non-specific background, active promoters, and repressed chromatin, respectively. Libraries were prepared using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001). Sequencing was performed with paired-end 50 bp reads, and data were processed on CUTANA™ Cloud (cloud.epicypher.com) by alignment to the hg38 genome. Images were generated using Integrative Genomics Viewer (IGV, Broad Institute).

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

CUT&RUN profiling with c-Myc antibody demonstrates robust genome-wide enrichment in wild-type (WT) cells, which is markedly diminished in MYC knockout (KO) cells. Heatmaps of genome-wide signal flanking annotated transcription start sites (TSSs, +/- 2 kbp) display CUT&RUN data generated using the CUTANA™ CUT&RUN Kit (EpiCypher 14-1048) with c-Myc antibody (Abcam ab32072, 0.5 µg). 500,000 HEK293T WT or KO (Abcam ab256500) cells were used per reaction. IgG antibody was included as a negative control to assess non-specific background. Libraries were prepared using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001). Sequencing was performed with paired-end 50 bp reads, and data were processed on CUTANA™ Cloud (cloud.epicypher.com) by alignment to the hg38 genome. Heatmaps were generated using deepTools (Ramнrez et al., Nucleic Acids Res. 2014; PMID : 24799436). Row-linked data are ranked by intensity relative to c-Myc WT, with red indicating high localized enrichment and blue denoting background. Validated antibodies show genome-wide enrichment above IgG background consistent with c-Myc binding in WT cells and near complete loss of signal in KO cells.

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

CUT&RUN profiling with c-Myc antibody reveals the expected genomic enrichment pattern in wild-type (WT) cells, which is substantially reduced in MYC knockout (KO) cells. Representative genome browser tracks show CUT&RUN data generated using the CUTANA™ CUT&RUN Kit (EpiCypher 14-1048) with c-Myc antibody (Abcam ab185656, 0.5 µg). 500,000 HEK293T WT or KO (Abcam ab256500) cells were used per reaction. IgG, H3K4me3, and H3K27me3 antibodies were included as controls to assess non-specific background, active promoters, and repressed chromatin, respectively. Libraries were prepared using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001). Sequencing was performed with paired-end 50 bp reads, and data were processed on CUTANA™ Cloud (cloud.epicypher.com) by alignment to the hg38 genome. Images were generated using Integrative Genomics Viewer (IGV, Broad Institute).

Sandwich ELISA - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • sELISA

Lab

Sandwich ELISA - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

Interpolated concentrations of native c-Myc in human control wild type HEK293T cell and MYC knockout HEK293T cell based on 1000 µg/mL extract loads. The concentrations of c-Myc were measured in duplicate and interpolated from the c-Myc standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean c-Myc concentration was determined to be 4509.9 pg/mL in wild type HEK293T extract (Human wild-type HEK293T cell line ab255449) and undetectable in MYC knockout HEK293T extract (Human MYC knockout HEK293T cell line ab256500).

Sanger Sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)
  • Sanger seq

Unknown

Sanger Sequencing - Human MYC (c-Myc) knockout HEK-293T cell line (AB256500)

Homozygous : 1 bp insertion in exon 2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Form

Liquid

form

Knockout validation

Immunohistochemistry,Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous (4N): 1bp T insertion (2N); 8 bp deletion and C to T insertion (1N); 4 bp deletion in exon 2 (1N)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ChIC/CUT&RUN-seq": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This cell line was generated with a puromycin resistance gene integrated at the gRNA cutting site, indicating it could still be expressed.

We will provide viable cells that proliferate on revival.

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
MYC
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Immunohistochemistry, Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C
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Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium

DMEM (High Glucose) + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.
Biological function summary

C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.

Pathways

C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.

C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Target data

See full target information MYC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 52:11587-11601 PubMed39315698

2024

Chem-CRISPR/dCas9FCPF: a platform for chemically induced epigenome editing.

Applications

Unspecified application

Species

Unspecified reactive species

Mukaddes Altinbay,Jianhui Wang,Jie Chen,Daniel Schäfer,Maximilian Sprang,Biljana Blagojevic,Stefan Wölfl,Miguel A Andrade-Navarro,Ivan Dikic,Stefan Knapp,Xinlai Cheng
View all publications
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