Human NARF (IOP2) knockout HeLa cell line
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NARF KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 3 and 1 bp deletion in exon 3 and 5 bp deletion in exon 3.
View Alternative Names
DKFZp434G0420, FLJ10067, GC17P078009, IOP2, Iron only hydrogenase like protein 2, NARF, Nuclear prelamin A recognition factor, Prenyl dependent prelamin A binding protein
- Sanger seq
Unknown
Sanger Sequencing - Human NARF (IOP2) knockout HeLa cell line (AB265489)
Allele-1 : 16 bp deletion in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human NARF (IOP2) knockout HeLa cell line (AB265489)
Allele-3 : 1 bp deletion in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human NARF (IOP2) knockout HeLa cell line (AB265489)
Allele-2 : 5 bp deletion in exon 3.
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IOP2 functions in intracellular signaling and membrane dynamics. It participates as a component of the ESCRT (endosomal sorting complexes required for transport) machinery. In this context IOP2 facilitates the sorting of ubiquitinated transmembrane proteins into the internal vesicles of multivesicular bodies. Its activity in this complex highlights the regulation of down-regulating surface receptors and contributing to the cellular homeostasis.
Pathways
IOP2 interacts with mechanisms controlling cellular growth and apoptosis. One of the significant pathways includes the mitogen-activated protein kinase (MAPK) signaling which influences cell proliferation. Within this pathway IOP2 relates to proteins such as ERK1/2 playing a role in the signal transduction processes. Additionally the protein contributes to the regulation of PI3K/AKT pathway impacting cell survival through its interactions within the signaling network.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com