Human NCK1 (Nck) knockout HeLa cell line
- Advanced Validation
- What is this?
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NCK1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
View Alternative Names
Cytoplasmic protein NCK1, MGC12668, Melanoma Nck protein, NCK, NCK adaptor protein 1, NCK alpha, NCK tyrosine kinase, NCK1_HUMAN, Non catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK-alpha
- WB
Lab
Western blot - Human NCK1 (Nck) knockout HeLa cell line (AB265323)
Lanes 1-3 : Merged signal (red and green). Green - ab32120 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa.
ab32120 Anti-Nck antibody [Y531] was shown to specifically react with Nck in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265323 (knockout cell lysate ab258059) was used. Wild-type and Nck knockout samples were subjected to SDS-PAGE. ab32120 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Nck antibody [Y531] (<a href='/en-us/products/primary-antibodies/nck-antibody-y531-ab32120'>ab32120</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
NCK1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human NCK1 (Nck) knockout HeLa cell line (ab265323)
Lane 3:
NIH/3T3 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human NCK1 (Nck) knockout HeLa cell line (AB265323)
Homozygous : 1 bp insertion in exon 2.
Reactivity data
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Nck proteins play significant roles in actin cytoskeleton reorganization influencing cell shape movement and adhesion. These proteins often participate as part of larger signaling complexes where they serve as scaffolds bringing together key molecules. Nck proteins directly interact with partners containing SH2 and SH3 domains which are important for various cellular dynamic processes. Their role in cytoskeletal dynamics aligns them with many cellular mechanisms including endocytosis and cell motility.
Pathways
Nck proteins significantly influence the regulation of the actin cytoskeleton pathway and the Rho GTPases pathway. Through these pathways they interact with proteins such as WASP and Rac regulating actin polymerization and cell migration. Nck's ability to form complex signaling networks positions it as a central actor in transducing signals that affect cell shape and movement. It acts collaboratively with multiple signaling pathways to maintain cellular plasticity and response to extracellular signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
![Flow Cytometry (Intracellular) - Anti-Nck antibody [Y531] (AB32120)](https://content.abcam.com/products/images/nck-antibody-y531-ab32120--flow-cytometry-intracellular-img79381.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com