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NCOA7 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 11.

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Sanger Sequencing - Human NCOA7 knockout A549 cell line (AB267144), expandable thumbnail

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Form

Liquid

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 11

Alternative names

Recommended products

NCOA7 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 11.

Key facts

Cell type

A549

Form

Liquid

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 11

Disease

Carcinoma

Concentration
Loading...

Properties

Gene name

NCOA7

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing

Zygosity

Homozygous

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines

  • All seeding densities should be based on cell counts gained by established methods.

  • A guide seeding density of 2x104 cells/cm2 is recommended.

  • Cells should be passaged when they have achieved 80-90% confluence.

  • Do not allow the cell density to exceed 7x104 cells/cm2.

Culture medium

F-12K + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-196°C

Appropriate long-term storage conditions

-196°C

Notes

Recommended control: Human wild-type A549 cell line (Human wild-type A549 cell line ab255450). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Nuclear receptor coactivator 7 (NCOA7) also known as ERAP plays a role in transcriptional regulation by interacting with nuclear hormone receptors to enhance their transcriptional activity. It has a molecular mass of approximately 100 kDa. NCOA7 is expressed in various tissues with significant levels in the brain and endocrine organs. The protein influences the transcriptional activity of hormone receptors by modulating chromatin structure and accessibility.

Biological function summary

NCOA7 influences cellular processes through interactions with nuclear receptors. It forms a complex with these receptors to modulate gene expression. This protein engages in the regulation of genes that are involved in cell development and homeostasis. NCOA7's activity can influence metabolic and signaling processes that are critical for maintaining proper cellular environment and function.

Pathways

NCOA7 associates with important biological pathways including nuclear hormone receptor signaling and lipid metabolism. It interacts with proteins like peroxisome proliferator-activated receptors (PPARs) and estrogen receptors (ERs) in these pathways. These interactions facilitate the regulation of genes involved in lipid storage energy balance and other metabolic functions.

Associated diseases and disorders

NCOA7 has implications in conditions such as breast cancer and neurodegenerative diseases. In breast cancer its interaction with estrogen receptors can influence tumor growth and development. NCOA7 is also connected to conditions like Alzheimer's disease through its influence on brain tissues potentially interacting with proteins like amyloid-beta precursor protein which is relevant to the disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sanger Sequencing - Human NCOA7 knockout A549 cell line (ab267144), expandable thumbnail

    Sanger Sequencing - Human NCOA7 knockout A549 cell line (ab267144)

    Homozygous: 1 bp deletion in exon11

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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