Human NCR3LG1 knockout Raji cell line
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NCR3LG1 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
B7 homolog 6, B7-H6, B7H6_HUMAN, DKFZp686O24166, NCR3LG1, Natural cytotoxicity triggering receptor 3 ligand 1, Putative Ig like domain containing protein DKFZp686O24166/DKFZp686I21167
- NGS
Lab
Next Generation Sequencing - Human NCR3LG1 knockout Raji cell line (AB290414)
176 bp deletion after Ile63 of the WT protein
- WB
Lab
Western blot - Human NCR3LG1 knockout Raji cell line (AB290414)
Western blot : Anti-B7-H6 antibody [EPR23470-210] ab253180 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 70-80 kDa in Wild-type Raji UNBOILED cell lysates with no signal observed at this size in NCR3LG1 knockout Raji UNBOILED cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween™ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes:
Western blot - Anti-B7-H6 antibody [EPR23470-210] (<a href='/en-us/products/primary-antibodies/b7-h6-antibody-epr23470-210-ab253180'>ab253180</a>) at 1/1000 dilution
Lane 1:
Wild-type Raji UNBOILED whole cell lysate at 20 µg
Lane 2:
Western blot - Human NCR3LG1 knockout Raji cell line (ab290414) at 20 µg
Lane 3:
HepG2 UNBOILED whole cell lysate at 20 µg
Lane 4:
K562 UNBOILED whole cell lysate at 20 µg
Lane 5:
Daudi UNBOILED whole cell lysate at 20 µg
Lane 6:
SK-OV-3 UNBOILED whole cell lysate at 20 µg
Secondary
All lanes:
Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 70-80 kDa
false
- WB
Lab
Western blot - Human NCR3LG1 knockout Raji cell line (AB290414)
Western blot : Anti-B7-H6 antibody [EPR21841] ab219205 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 70-80 kDa in Wild-type Raji UNBOILED cell lysates with no signal observed at this size in NCR3LG1 knockout Raji UNBOILED cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween™ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes:
Western blot - Anti-B7-H6 antibody [EPR21841] (<a href='/en-us/products/primary-antibodies/b7-h6-antibody-epr21841-ab219205'>ab219205</a>) at 1/1000 dilution
Lane 1:
Wild-type Raji UNBOILED whole cell lysate at 20 µg
Lane 2:
Western blot - Human NCR3LG1 knockout Raji cell line (ab290414) at 20 µg
Lane 3:
HepG2 UNBOILED whole cell lysate at 20 µg
Lane 4:
K562 UNBOILED whole cell lysate at 20 µg
Lane 5:
Daudi UNBOILED whole cell lysate at 20 µg
Lane 6:
SK-OV-3 UNBOILED whole cell lysate at 20 µg
Secondary
All lanes:
Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 70-80 kDa
false
Reactivity data
Product details
Recommended control: Human wild-type Raji cell line (ab290717). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 4x105 - 5x105 cells/mL(for initial passages it is recomended to culture the cells in the higher range of recomended seeding density). Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 4x105 cells/mL is recommended.
- A maximum of 3x106 viable cells/mL is obtainable.
Culture medium
RPMI + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
B7-H6 acts as an important component in natural killer cell-mediated cytotoxicity. The protein is not part of a larger complex but operates independently to engage NK cell activity. It serves as a signal for NK cells to identify and destroy tumor cells. Additionally B7-H6 expression on tumor cells often correlates with immune cell recognition thereby playing an important role in tumor immunity.
Pathways
B7-H6 participates in the immune response pathways connected to natural killer cell activation. Specifically it interacts with proteins like NKp30 as part of the natural cytotoxicity triggering receptor signaling process. These interactions facilitate the destruction of target cells enhancing the body's innate immune response by activating downstream pathways vital to immune defense.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Suspension
Gender
Male
Target data
Complementary Products
Primary Antibodies
AB253180
Anti-B7-H6 antibody [EPR23470-210]
RabMAb|Recombinant|KO Validated|Trial Size|Trial Size|Trial Size
![Immunoprecipitation - Anti-B7-H6 antibody [EPR23470-210] (AB253180)](https://content.abcam.com/products/images/b7-h6-antibody-epr23470-210-ab253180--immunoprecipitation-img8873.jpg)
- 0
- 0
Primary Antibodies
AB283657
Alexa Fluor® 647 Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139]
RabMAb|Recombinant
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (AB283657)](https://content.abcam.com/products/images/alexa-fluor-647-kir3dl1-kir2ds4-kir2ds2-kir3ds1-antibody-epr24357-139-ab283657--immunocytochemistry-immunofluorescence-img172479.jpg)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com