NDUFA7 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 1.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 1
Alternative names
B14.5a, CI-B14.5a, Complex I-B14.5a, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 7 14.5kDa, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7, NADH-ubiquinone oxidoreductase subunit B14.5a, NDUA7_HUMAN
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NDUFA7 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 1
- Concentration
Properties
- Gene name
- NDUFA7
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NDUFA7 protein also known as NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 is an integral part of the mitochondrial respiratory chain. It has a molecular weight of approximately 12 kDa. This protein is expressed mostly in the mitochondria of various cells and tissues such as muscle liver and brain. It plays a significant role in the electron transport chain where it contributes to the transfer of electrons from NADH to ubiquinone therefore contributing to ATP production.
- Biological function summary
The NDUFA7 protein operates as a component of the mitochondrial complex I also referred to as NADH:ubiquinone oxidoreductase. This complex which includes around 45 different subunits is the first and largest enzyme of the mitochondrial respiratory chain. NDUFA7 does not directly catalyze the electron transfer but stabilizes the structure of the complex necessary for efficient electron flow. Proper functioning of complex I is essential for maintaining cellular energy homeostasis and normal metabolic activity.
- Pathways
NDUFA7 plays an important role within the oxidative phosphorylation pathway which is important for aerobic energy production. This protein is closely related to other proteins in complex I such as NDUFA9 which together ensure efficient functioning of the electron transfer process. Moreover NDUFA7 indirectly influences other metabolic networks that rely on ATP including the TCA cycle and fatty acid oxidation highlighting its fundamental contribution to cellular metabolism.
- Associated diseases and disorders
Mutations in the NDUFA7 gene can lead to mitochondrial disorders such as Leigh syndrome and mitochondrial complex I deficiency. These are serious conditions that may result in motor disability developmental challenges and other systemic problems. In the context of these disorders NDUFA7 interacts with proteins like NDUFS1 which also constitutes a part of complex I and defects in these proteins can amplify mitochondrial dysfunction highlighting the interconnectedness of protein actions within the cell.
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1 product image
Sanger Sequencing - Human NDUFA7 knockout HEK-293T cell line (ab266597)
Homozygous: 8 bp deletion in exon 1
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