NLGN4X KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2
Alternative names
ASPGX2, AUTSX2, HLNX, HNL4X, HNLX, KIAA1260, NL4, NLGN, NLGN4X, NLGNX_HUMAN, Neuroligin X, Neuroligin-4, OTTHUMP00000022863, OTTHUMP00000022864, OTTHUMP00000022865, X-linked, neuroligin 4, X-linked
Recommended products
NLGN4X KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- NLGN4X
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NLGN4X also known as neuroligin 4X is a protein that plays a critical role in synaptic function and signaling in the human brain. This protein with a molecular mass of approximately 91 kDa is part of the neuroligin family which includes several proteins integral to the formation and maintenance of synapses. It is predominantly expressed in the central nervous system particularly in areas where neuronal communication is essential. NLGN4X assists in the important task of cell–cell interaction at synaptic junctions promoting synaptic stability and ensuring efficient neurotransmission.
- Biological function summary
NLGN4X participates in the maintenance of synaptic function both in excitatory and inhibitory synapses. It forms part of a larger synaptic complex that includes neurexins and other proteins essential for synaptic adhesion and signal transduction. These complexes are necessary for precise neurotransmitter release and reception allowing neurons to reliably and efficiently communicate. The structural integrity provided by NLGN4X's presence in these complexes underlies normal synaptic operation and influences cognitive processes.
- Pathways
Neuroligins belong to multiple neurological pathways emphasizing their role in synaptic organization and plasticity. NLGN4X engages with pathways like the synaptic vesicle cycle and postsynaptic density dynamics vital for modulating synaptic strength and plasticity. Through these pathways NLGN4X interacts closely with proteins such as neurexins facilitating synaptic organization and communication that are fundamental in neural network function.
- Associated diseases and disorders
Alterations in NLGN4X are strongly associated with autism spectrum disorders and intellectual disability. Mutations or deletions within the NLGN4X gene can disrupt normal synaptic connectivity leading to impaired social behavior and cognitive functions inherent in these disorders. NLGN4X is intertwined with other proteins such as SHANK3 both linked to synaptic stability and function. Disruption in their interactions can exacerbate synaptic and functional deficits observed in these conditions.
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2 product images
Sanger Sequencing - Human NLGN4X knockout HeLa cell line (ab264783)
Allele-2: Insertion of the selection cassette in exon 2.
Sanger Sequencing - Human NLGN4X knockout HeLa cell line (ab264783)
Allele-1: 1 bp deletion in exon 2.
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