Human NLRC5 knockout A549 cell line
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- Sanger seq
Unknown
Sanger Sequencing - Human NLRC5 knockout A549 cell line (AB266987)
Allele-2 : 8 bp deletion in exon 5.
- Sanger seq
Unknown
Sanger Sequencing - Human NLRC5 knockout A549 cell line (AB266987)
Allele-1 : 13 bp deletion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human NLRC5 knockout A549 cell line (AB266987)
Allele-3 : 1 bp insertion in exon 5.
Complementary Products
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
Culture medium
F-12K + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NLRC5 engages in enhancing immune surveillance and antiviral responses by regulating MHC class I transcription. It acts as part of the larger NLR family of proteins which are known to participate in the immune system's response to pathogens. In addition to its role in the immune response NLRC5 can interact with components of the inflammasome complex although its presence in the inflammasome remains controversial. These mechanisms are essential in initializing and sustaining an immune response against infections.
Pathways
NLRC5 involves itself in the interferon signaling pathway and antigen processing and presentation pathways. In the context of interferon signaling NLRC5 influences the activation of genes that drive antiviral states by interacting with proteins such as IRF1 and STAT1. This regulatory role positions NLRC5 as a bridge between innate and adaptive immune responses ensuring proper signaling and presentation of antigens to T cells for effective immune resolution.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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