NMT1 KO cell line available now. Free of charge wild type control available.
Jurkat
Human
Blood
Liquid
Alternative, short form NMT S, EC 2.3.1.97, Glycylpeptide N-tetradecanoyltransferase 1, Long form, NMT L, Myristoyl-CoA:protein N-myristoyltransferase 1, N myristoyltransferase 1, NMT, NMT1_HUMAN, Peptide N-myristoyltransferase 1, Type I N-myristoyltransferase
NMT1 KO cell line available now. Free of charge wild type control available.
Alternative, short form NMT S, EC 2.3.1.97, Glycylpeptide N-tetradecanoyltransferase 1, Long form, NMT L, Myristoyl-CoA:protein N-myristoyltransferase 1, N myristoyltransferase 1, NMT, NMT1_HUMAN, Peptide N-myristoyltransferase 1, Type I N-myristoyltransferase
Jurkat
Human
Blood
Liquid
Non-Hodgkin Lymphoma
NMT1
Knockout
CRISPR technology
EU: 1 US: 1
~ 80%
Suspension
Male
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
RPMI + 10% FBS
Dry Ice
-196°C
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type Jurkat cell line (Human wild-type Jurkat cell line ab275468). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture medium: RPMI + 10% FBS
Initial handling guidelines: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
Subculture guidelines:
• All seeding densities should be based on cell counts gained by established methods.
• A guide seeding density of 2x105 cells/mL is recommended.
•Do not allow the cell density to exceed 3x106 cells/mL
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
This supplementary information is collated from multiple sources and compiled automatically.
N-myristoyltransferase 1 often referred to as NMT1 or NMT is an enzyme essential for catalyzing the transfer of myristate a 14-carbon saturated fatty acid to the N-terminal glycine residue of substrate proteins. This post-translational modification is called myristoylation and is necessary for the proper functioning of many proteins. NMT1 has a molecular mass of approximately 50 kDa and is widely expressed in eukaryotic cells prominently in the cytoplasm and sometimes associated with intracellular membranes.
NMT1 modifies proteins by attaching myristic acid influencing their membrane-binding capability stability and function. It does not form part of a larger complex but rather acts individually to regulate protein activity through myristoylation. This modification is important for the localization and activation of proteins involved in various cellular processes including signal transduction and apoptosis.
N-myristoyltransferase 1 plays a role in several key biological pathways. Myristoylation mediated by NMT1 regulates signal transduction pathways such as the Src family kinases and G-protein signaling. Through these pathways NMT1 interacts with key proteins like Src a tyrosine kinase involved in cell growth and differentiation and Gα proteins which are part of the G-protein coupled receptor signaling pathway.
Alterations in NMT1 activity are associated with various cancers and infectious diseases. For instance overexpression of NMT1 has been observed in colorectal cancer potentially linked through its interactions with cancer-related proteins like Src. Additionally the enzyme is important for the survival of certain pathogens which require myristoylation for their life cycle suggesting that NMT1 could be a target for antimicrobial drug development.
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