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NMT1 KO cell line available now. Free of charge wild type control available.

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Key facts

Cell type

Jurkat

Species or organism

Human

Tissue

Blood

Form

Liquid

Alternative names

Recommended products

NMT1 KO cell line available now. Free of charge wild type control available.

Alternative names

Key facts

Cell type

Jurkat

Form

Liquid

Disease

Non-Hodgkin Lymphoma

Concentration
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Properties

Gene name

NMT1

Gene editing type

Knockout

Gene editing method

CRISPR technology

Cell culture

Biosafety level

EU: 1 US: 1

Viability

~ 80%

Adherent/suspension

Suspension

Gender

Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.

Subculture guidelines

  • All seeding densities should be based on cell counts gained by established methods.

  • A guide seeding density of 2x105 cells/mL is recommended.

  • Do not allow cell density to exceed 3x106 cells/mL.

Culture medium

RPMI + 10% FBS

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-196°C

Notes

Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.

Recommended control: Human wild-type Jurkat cell line (Human wild-type Jurkat cell line ab275468). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Culture medium:  RPMI + 10% FBS

Initial handling guidelines: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.

Subculture guidelines:
• All seeding densities should be based on cell counts gained by established methods.
• A guide seeding density of 2x105 cells/mL is recommended.
•Do not allow the cell density to exceed 3x106 cells/mL

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

N-myristoyltransferase 1 often referred to as NMT1 or NMT is an enzyme essential for catalyzing the transfer of myristate a 14-carbon saturated fatty acid to the N-terminal glycine residue of substrate proteins. This post-translational modification is called myristoylation and is necessary for the proper functioning of many proteins. NMT1 has a molecular mass of approximately 50 kDa and is widely expressed in eukaryotic cells prominently in the cytoplasm and sometimes associated with intracellular membranes.

Biological function summary

NMT1 modifies proteins by attaching myristic acid influencing their membrane-binding capability stability and function. It does not form part of a larger complex but rather acts individually to regulate protein activity through myristoylation. This modification is important for the localization and activation of proteins involved in various cellular processes including signal transduction and apoptosis.

Pathways

N-myristoyltransferase 1 plays a role in several key biological pathways. Myristoylation mediated by NMT1 regulates signal transduction pathways such as the Src family kinases and G-protein signaling. Through these pathways NMT1 interacts with key proteins like Src a tyrosine kinase involved in cell growth and differentiation and Gα proteins which are part of the G-protein coupled receptor signaling pathway.

Associated diseases and disorders

Alterations in NMT1 activity are associated with various cancers and infectious diseases. For instance overexpression of NMT1 has been observed in colorectal cancer potentially linked through its interactions with cancer-related proteins like Src. Additionally the enzyme is important for the survival of certain pathogens which require myristoylation for their life cycle suggesting that NMT1 could be a target for antimicrobial drug development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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