NOMO2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1 and 2 bp insertion in exon 1.
Images
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Form
Liquid
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1 and 2 bp insertion in exon 1
Alternative names
NOMO2_HUMAN, Nodal modulator 2, pM5 protein 2
Recommended products
NOMO2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1 and 2 bp insertion in exon 1.
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Form
Liquid
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1 and 2 bp insertion in exon 1
- Disease
Adenocarcinoma
- Concentration
Properties
- Gene name
NOMO2
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Sanger Sequencing
Quality control
- STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
EU: 2 US: 2
- Adherent/suspension
Adherent
- Gender
Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-196°C
- Appropriate long-term storage conditions
-196°C
Notes
Recommended control: Human wild-type HeLa cell line (Human wild-type HeLa cell line ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NOMO2 also known as Nodal Modulator 2 or pM5 is an endoplasmic reticulum membrane protein with a molecular mass of approximately 130 kDa. It functions mechanically as a part of the Nomo family which includes homologous proteins such as NOMO1 and NOMO3. The NOMO2 protein plays a critical role in the suppression of Nodal signaling. This protein is ubiquitously expressed but with higher levels observed in tissues including the pancreas and testis.
- Biological function summary
NOMO2 modulates essential signaling pathways during early embryonic development. It acts as part of a larger protein complex known as the Nicalin-NOMO complex which modulates Nodal signaling. This signaling is important for pattern formation and axis specification during embryogenesis. By participating in these processes NOMO2 contributes to the regulation of cellular differentiation and proliferation.
- Pathways
NOMO2 operates within the TGF-beta signaling pathway an important player in cell growth and differentiation. It directly influences Nodal signaling a branch of the TGF-beta pathway by interacting with other proteins like Nicalin and TMEM147. Through its involvement in these pathways NOMO2 helps coordinate cellular responses and genetic transcription important for embryonic patterning and organogenesis.
- Associated diseases and disorders
NOMO2 is linked to congenital disorders resulting from disrupted embryonic development. Alterations in NOMO2 function can contribute to conditions such as congenital heart defects. Additionally NOMO2's connection with the TGF-beta pathway may influence cancer progression as this pathway frequently interacts with oncogenes and tumor suppressors like SMAD proteins that are dysregulated in cancer.
Product promise
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3 product images
Sanger Sequencing - Human NOMO2 knockout HeLa cell line (ab265218)
Allele-3: 2 bp insertion in exon 1.
Sanger Sequencing - Human NOMO2 knockout HeLa cell line (ab265218)
Allele-2: 1 bp insertion in exon 1.
Sanger Sequencing - Human NOMO2 knockout HeLa cell line (ab265218)
Allele-1: 1 bp deletion in exon 1.
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