NPR1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1
Alternative names
ANP-A, ANPRA_HUMAN, Atrial natriuretic peptide A type receptor, Atrial natriuretic peptide receptor 1, Atrial natriuretic peptide receptor A, Atrial natriuretic peptide receptor type A, Atrionatriuretic peptide receptor A, GC-A, GUCY2A, Guanylate cyclase, Guanylate cyclase A, NPR-A, NPR1, Natriuretic Peptide Receptor A, Natriuretic peptide A type receptor, Natriuretic peptide receptor A/guanylate cyclase A
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NPR1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- NPR1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NPR-A also known as natriuretic peptide receptor A is a guanylyl cyclase-linked receptor with a mass of approximately 120 kDa. It largely expresses in the kidney heart adrenal gland and vascular smooth muscle cells. NPR-A is structured to bind specifically with atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) facilitating signal transduction. This receptor generates cyclic GMP (cGMP) upon activation which acts as a secondary messenger in various cellular processes.
- Biological function summary
Natriuretic peptide receptor A plays a significant role in cardiovascular homeostasis. NPR-A is not part of a larger complex but works closely with other natriuretic peptide systems to regulate blood pressure electrolyte balance and fluid homeostasis. Its activation results in diuresis natriuresis and vasodilation contributing to its function in maintaining cardiovascular health. Efficient functioning of NPR-A is essential for modulating cardiovascular responses.
- Pathways
NPR-A is important in the cyclic GMP pathway and the ANP signaling pathway. It interacts with proteins like NPR-C and soluble guanylyl cyclase modulating key physiological responses. NPR-A impacts smooth muscle relaxation and renal function by influencing cGMP levels and related downstream pathways. Its role within these pathways is fundamental to maintaining vascular tone and sodium excretion.
- Associated diseases and disorders
NPR-A associates prominently with cardiovascular diseases such as hypertension and heart failure. In hypertension NPR-A dysregulation can impair proper sodium and water excretion exacerbating the condition. Heart failure involves altered signaling of NPR-A impacting cardiac and renal function. Protein interactions include altered expression of NPR-C affecting natriuretic peptide clearance and disease progression. Understanding NPR-A's function and regulation provides insights into its relationship with cardiovascular pathophysiology and potential therapeutic approaches.
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2 product images
Sanger Sequencing - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line (ab265930)
Allele-2: 1 bp insertion in exon 1.
Sanger Sequencing - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line (ab265930)
Allele-1: 5 bp deletion in exon 1.
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