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NR2C2 KO cell line available now. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 6 and Insertion of the selection cassette in exon 6.
Alternative names=NR2C2_HUMAN, Nuclear hormone receptor TR4, Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, TAK1, TR2R1, TR4 nuclear hormone receptor, Testicular nuclear receptor 4, Testicular receptor 4, hTAK1
- Cell type
HEK-293T
- Species or organism
Human
- Tissue
Kidney
- Form
Liquid
- Knockout validation
Sanger Sequencing
Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 6 and Insertion of the selection cassette in exon 6
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NR2C2 KO cell line available now. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 6 and Insertion of the selection cassette in exon 6.
Alternative names=NR2C2_HUMAN, Nuclear hormone receptor TR4, Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, TAK1, TR2R1, TR4 nuclear hormone receptor, Testicular nuclear receptor 4, Testicular receptor 4, hTAK1
Key facts
- Cell type
HEK-293T
- Species or organism
Human
- Tissue
Kidney
- Form
Liquid
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 6 and Insertion of the selection cassette in exon 6
- Concentration
Properties
- Gene name
NR2C2
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Sanger Sequencing
Quality control
- STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
EU: 2 US: 2
- Adherent/suspension
Adherent
- Gender
Female
Handling procedures
- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-196°C
Notes
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
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