Lanes 1 - 4: Merged signal (red and green). Green - ab183127 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab183127 was shown to recognize NR3C1 in wild-type A549 cells as signal was lost at the expected MW in NR3C1 knockout cell line ab261862 (knockout cell lysate ab261671). Additional cross-reactive bands were observed in the wild-type and knockout samples. Wild-type and NR3C1 knockout samples were subjected to SDS-PAGE. Ab183127 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

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