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AB306847

Human NRP2 knockout U-87 MG cell line

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NRP2 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
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Next Generation Sequencing - Human NRP2 knockout U-87 MG cell line (AB306847)
  • NGS

Supplier Data

Next Generation Sequencing - Human NRP2 knockout U-87 MG cell line (AB306847)

40 bp deletion in exon 4, CCDS46496.1

Key facts

Cell type

U-87 MG

Species or organism

Human

Tissue

Brain

Form

Liquid

form

Knockout validation

Next Generation Sequencing

Disease

Glioblastoma

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Complementary Products

Proteins & Peptides

AB277019

Recombinant mouse NRP2 protein (His tag)

Functional Studies - Recombinant mouse NRP2 protein (His tag) (AB277019)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "NGS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
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What's included?

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Properties and storage information

Gene name
NRP2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C
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Handling procedures

Initial handling guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium

EMEM + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NRP2 also known as Neuropilin-2 is a receptor protein involved in developmental processes including axon guidance and angiogenesis. The molecular mass of NRP2 is approximately 120 kDa. It is expressed in various tissues such as the nervous system and the vascular system but its expression can also be found in certain immune cells. NRP2 plays an important role in cellular signaling by binding to semaphorins and vascular endothelial growth factors (VEGF).
Biological function summary

NRP2 influences several processes including axonal guidance and the formation of blood vessels. It acts as a co-receptor by forming complexes with other proteins to mediate cellular responses. This role in signaling helps guide neuron growth and regulates vascular development. The NRP2-associated complexes often modulate responses to external signals contributing to the precise control of network formation in tissues.

Pathways

NRP2 plays a significant role in the VEGF signaling pathway and the semaphorin signaling pathway. These pathways are essential for cell growth migration and differentiation. NRP2 works closely with VEGF receptors like VEGFR2 to promote angiogenic responses. It also interacts with semaphorin family proteins to guide neuronal development. Together these pathways ensure the proper formation and function of neural and vascular networks.

NRP2 is connected to cancer progression and cardiovascular diseases. Altered expression of NRP2 has been observed in various cancer types where it may contribute to tumor growth and metastasis. In these diseases NRP2's interaction with its partners such as VEGFR2 becomes dysregulated affecting normal cellular processes. Cardiovascular conditions can also arise from issues in NRP2-related signaling where the normal function of blood vessel formation is compromised.
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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Target data

See full target information NRP2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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