Human NSUN2 (SAKI) knockout HEK-293T cell line
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NSUN2 KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 23 bp deletion in exon 2 and 32 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
View Alternative Names
5 methycytoisine methyltransferase, D13Wsu123e, FLJ20303, MISU, Myc induced SUN domain containing protein, NOL1/NOP2/Sun domain family 2, NOL1/NOP2/Sun domain family 2 protein, NOL1/NOP2/Sun domain family member 2, NSUN2_HUMAN, SAKI, Substrate of AIM1/Aurora kinase B, TRM4, hTrm4, tRNA (cytosine 5 ) methyltransferase NSUN2, tRNA (cytosine(34)-C(5))-methyltransferase, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolo, tRNA methyltransferase 4 homolog
- Sanger seq
Unknown
Sanger Sequencing - Human NSUN2 (SAKI) knockout HEK-293T cell line (AB266273)
Allele-2 : 23 bp deletion in exon 2.
- Sanger seq
Unknown
Sanger Sequencing - Human NSUN2 (SAKI) knockout HEK-293T cell line (AB266273)
Allele-3 : Insertion of the selection cassette in exon 2.
- Sanger seq
Unknown
Sanger Sequencing - Human NSUN2 (SAKI) knockout HEK-293T cell line (AB266273)
Allele-1 : 32 bp deletion in exon 2
Product details
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NSUN2 participates in RNA metabolism and processing. By introducing m5C (5-methylcytosine) modifications to RNA NSUN2 influences RNA stability transport and translation. This protein is a part of larger complexes that regulate diverse cellular functions and maintains transcriptome integrity. Alterations in NSUN2 activity can affect cellular proliferation and differentiation highlighting its role in maintaining normal cell function.
Pathways
NSUN2 plays an integral role in the epitranscriptomic regulation pathway in particular RNA modification and gene expression regulation pathways. It acts alongside proteins like TRMT2A and TRMT61A which are involved in similar processes of RNA methylation and modification. These pathways help maintain a balance in gene expression profiles necessary for proper cellular function and response to external signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB259941
Anti-NSUN2/SAKI antibody [EPR24140-93]
BOND RX™ Validated|RabMAb|Recombinant
![Immunocytochemistry/ Immunofluorescence - Anti-NSUN2/SAKI antibody [EPR24140-93] (AB259941)](https://content.abcam.com/products/images/nsun2-saki-antibody-epr24140-93-ab259941--immunocytochemistry-immunofluorescence-img86565.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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