Human NTRK3 knockout U-87 MG cell line
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- NGS
Supplier Data
Next Generation Sequencing - Human NTRK3 knockout U-87 MG cell line (AB306744)
55 bp deletion after Val220 of the WT protein
Complementary Products
Primary Antibodies
AB240651
Anti-TrkC antibody [EPR22959-40]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkC antibody [EPR22959-40] (AB240651)](https://content.abcam.com/products/images/trkc-antibody-epr22959-40-ab240651--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img178528.jpg)
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Reactivity data
Product details
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
EMEM + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TrkC is critical for neuronal development and function. It is part of a signaling complex that mediates the effects of the neurotrophin-3 involved in the survival differentiation and maintenance of various neuron populations. TrkC activation leads to the phosphorylation of the receptor and downstream signaling molecules impacting cellular outcomes. It has important roles in nervous system development and in processes such as synaptic plasticity and memory formation.
Pathways
TrkC participates in neurotrophin signaling and MAPK pathways. These pathways regulate important cellular activities like growth survival and differentiation. In the context of neurotrophin signaling related proteins such as TrkA and TrkB share similar pathway components influencing similar biological processes. TrkC plays a pivotal role in modulating pathway-related cellular responses and integrating signals from NT-3 to execute proper neuronal functioning.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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