NUDT2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 16 bp insertion in exon 2 and 1 bp deletion in exon 2 and 8 bp deletion in exon 2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 16 bp insertion in exon 2 and 1 bp deletion in exon 2 and 8 bp deletion in exon 2
Alternative names
2310051L06Rik, 5''''-P1, AA939917, AP4A_HUMAN, APAH 1, Ap4A hydrolase, Ap4A hydrolase 1, Ap4Aase, Bis(5' nucleosyl) tetraphosphatase [asymmetrical], Bis(5''-nucleosyl)-tetraphosphatase [asymmetrical], Diadenosine 5'', Diadenosine 5',5''' P1,P4 tetraphosphate asymmetrical hydrolase, Diadenosine 5',5''-P1,P4-tetraphosphate pyrophosphohydrolase, Diadenosine tetraphosphatase, EC 3.6.1.17, MGC10404, Nucleoside diphosphate-linked moiety X motif 2, Nudix motif 2, Nudix-type motif 2, OTTHUMP00000021256, OTTHUMP00000021257, OTTHUMP00000021258, OTTHUMP00000021259, P4-tetraphosphate asymmetrical hydrolase, nudix (nucleoside diphosphate linked moiety X)-type motif 2
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NUDT2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 16 bp insertion in exon 2 and 1 bp deletion in exon 2 and 8 bp deletion in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 16 bp insertion in exon 2 and 1 bp deletion in exon 2 and 8 bp deletion in exon 2
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- NUDT2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein NUDT2 also known as Nucleoside Diphosphate-Linked Moiety X-Type Motif 2 is an enzyme that hydrolyzes nucleoside diphosphates. It has a molecular mass of approximately 22 kDa. This protein is expressed in various tissues including the brain liver and heart. NUDT2's mechanical function centers on its ability to regulate cellular nucleoside diphosphate levels by converting them into their monophosphate forms which is vital for the proper recycling of nucleotide pool components.
- Biological function summary
The protein plays a role in controlling intracellular hydrolysis of nucleoside diphosphates ensuring proper nucleotide balance. Although it operates independently it functions in synergy with other proteins involved in nucleotide metabolism. This precision in nucleotide regulation is essential for maintaining nucleic acid synthesis as well as energy transfer processes within the cell. NUDT2 does not form large complexes but interacts dynamically within metabolic hubs.
- Pathways
NUDT2 participates in nucleotide metabolism and salvage pathways. It contributes predominantly to pathways that recycle nucleotides essential for cellular replication and repair processes. Within these pathways NUDT2 closely interacts with enzymes such as nucleoside diphosphate kinases which play a complementary role in maintaining nucleotide balance. These interactions ensure efficient utilization of resources required for vital cellular functions.
- Associated diseases and disorders
NUDT2 has been linked to conditions such as cancer and metabolic disorders. Aberrant expression or dysfunction of NUDT2 can result in nucleotide imbalance leading to problems with cell proliferation or metabolic dysregulation. In certain cancers altered expression of NUDT2 in conjunction with disrupted functions of tumor suppressor proteins can contribute to uncontrolled cell growth. Understanding NUDT2's role in these diseases can guide therapeutic strategies aimed at restoring normal nucleotide metabolism.
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3 product images
Sanger Sequencing - Human NUDT2 knockout HeLa cell line (ab265752)
Allele-2: 1 bp deletion in exon 2.
Sanger Sequencing - Human NUDT2 knockout HeLa cell line (ab265752)
Allele-3: 16 bp insertion in exon 2.
Sanger Sequencing - Human NUDT2 knockout HeLa cell line (ab265752)
Allele-1: 8 bp deletion in exon 2.
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