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NUDT5 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.

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Images

Western blot - Human NUDT5 knockout HEK-293T cell line (AB266242), expandable thumbnail
  • Cell Culture - Human NUDT5 knockout HEK-293T cell line (AB266242), expandable thumbnail
  • Sanger Sequencing - Human NUDT5 knockout HEK-293T cell line (AB266242), expandable thumbnail

Key facts

Cell type
HEK-293T
Species or organism
Human
Tissue
Kidney
Form
Liquid
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2

Alternative names

ADP-sugar pyrophosphatase, NUDT5_HUMAN, Nucleoside diphosphate linked moiety X type motif 5, Nucleoside diphosphate-linked moiety X motif 5, Nudix (nucleoside diphosphate linked moiety X) type motif 5, Nudix motif 5, Nudix type motif 5, YSA1, YSA1H, hYSAH 1

Recommended products

NUDT5 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.

Key facts

Cell type
HEK-293T
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2
Concentration
Loading...

Properties

Gene name
NUDT5
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Handling procedures

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Nudix hydrolase 5 (NUDT5) enzyme also known as ADP-sugar pyrophosphatase performs important mechanical roles by hydrolyzing ADP-ribose to ribose-5-phosphate and AMP. It holds a molecular mass of approximately 39 kDa. NUDT5 shows high expression levels in tissues like liver and ovary. Its activity involves detoxifying nucleoside diphosphate derivatives which prevents their accumulation and potential interference with normal cellular processes.

Biological function summary

NUDT5 influences intracellular signaling pathways and DNA repair processes. It operates as part of multi-enzyme complexes that manage nucleotide metabolism and signaling. By maintaining a balance of ADP-ribose inside cells NUDT5 helps regulate cellular stress responses and genomic stability. Its role in transforming ADP-ribose supports maintaining adequate cellular energy and nucleotide pools for various biological functions.

Pathways

Research identifies NUDT5 as being involved in the ADP-ribose metabolism pathway and DNA repair mechanisms. In the ADP-ribose pathway NUDT5 assists in the regulation of ADP-ribose levels working alongside other proteins like PARP1 which adds ADP-ribose units to proteins during DNA repair. NUDT5's actions in these pathways ensure the proper functioning of cellular stress responses and the prevention of genome instability.

Associated diseases and disorders

NUDT5 has connections to tumorigenesis and neurodegenerative disorders. Elevated activity of NUDT5 is sometimes observed in certain cancers suggesting a potential role in cancer cell viability. In neurodegenerative diseases such as Parkinson's dysfunctional ADP-ribose metabolism implicates NUDT5. Changes in NUDT5 activity can interact with proteins like PARP1 altering cellular processes that contribute to disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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