OAS1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2
Alternative names
(2 5')oligo(A) synthetase 1, (2-5'')oligo(A) synthase 1, 2 5 Oligoadenylate Synthetase 1, 2 5' oligo A synthase 1, 2 5' oligo A synthetase 1, 2 5A synthetase 1, 2' 5' oligo A synthetase 1, 2' 5' oligoadenylate synthetase 1, 2' 5' oligoadenylate synthetase 1 40/46kDa, 2' 5' oligoisoadenylate synthetase 1, 2''-5''-oligoadenylate synthase 1, 2-5A synthase 1, E18/E16, OAS1_HUMAN, OIAS, OIASI, p46/p42 OAS
Recommended products
OAS1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- OAS1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
- Culture medium
- F-12K + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The OAS1 protein also known as 2'-5'-oligoadenylate synthetase 1 plays an important role in the antiviral response of the body. It has a molecular mass of approximately 42 kDa. This protein is expressed in various tissues including the liver lungs and heart. OAS1 is part of a family of interferon-induced enzymes that synthesize 2'-5'-linked oligoadenylates which are important for cellular defense mechanisms against viral infections.
- Biological function summary
The creation of 2'-5'-linked oligoadenylates by OAS1 is essential in activating RNase L an enzyme that degrades viral and cellular RNA to impede viral replication. OAS1 does not operate as part of a larger protein complex but functions independently to mediate antiviral activities. It has been observed that upon viral infection the levels of OAS1 increase enhancing the interferon response and cytokine signaling.
- Pathways
OAS1 plays a significant role in the interferon signaling pathway and innate immune response pathways. Within these pathways OAS1 interacts with other proteins such as RNase L and is also associated with STAT1 a critical mediator of the cellular response to interferons. The function of OAS1 within these pathways highlights its importance in modulating immune responses to pathogens.
- Associated diseases and disorders
OAS1 has been linked to conditions such as viral infections and autoimmune diseases. Its role in viral infections involves the detection and degradation of viral RNA thereby limiting viral proliferation. The interaction of OAS1 with RNase L is important in this process. In autoimmune disorders certain variants of OAS1 can alter immune function potentially contributing to abnormal immune responses and disease progression.
Product promise
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2 product images
Western blot - Human OAS1 knockout A549 cell line (ab267108)
False colour image of Western blot: Anti-OAS1 antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to OAS1. A band was observed at 40 kDa in treated wild-type A549 cell lysates with no signal observed at this size in OAS1 knockout cell line ab267108 (knockout cell lysate Human OAS1 knockout A549 cell lysate ab259011). To generate this image, wild-type and OAS1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
Performed under reducing conditions.
Sanger Sequencing - Human OAS1 knockout A549 cell line (ab267108)
Homozygous: 1 bp insertion in exon2
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