OAS3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 5 bp deletion in exon 1.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 5 bp deletion in exon 1
Alternative names
(2-5'')oligo(A) synthase 3, 2 5' oligo(A) synthetase 3, 2 5'LIGO, 2 5A synthetase 3, 2''-5''-oligoadenylate synthase 3, 2-5A synthase 3, MGC133260, OAS3 2' 5' oligoadenylate synthetase 3, 100kDa, OAS3_HUMAN, p100 OAS
Recommended products
OAS3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 5 bp deletion in exon 1.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 5 bp deletion in exon 1
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- OAS3
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
- Culture medium
- F-12K + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 2'-5'-oligoadenylate synthetase 3 (OAS3) protein also known as OAS03 OAS-treate or simply OAS003 plays an important role in the innate immune response to viral infections. OAS3 is a member of the 2'-5'-oligoadenylate synthetase family and possesses a mass of approximately 125 kDa. It is ubiquitously expressed in various tissues but shows higher expression levels in liver lung and spleen. This protein is involved in the synthesis of 2'-5'-linked oligoadenylates from ATP an activity which is important for antiviral defense mechanisms in the cell.
- Biological function summary
OAS3 contributes to the host's defense by activating RNase L a ribonuclease that degrades viral and cellular RNA to limit viral replication. OAS3 functions as part of the larger oligoadenylate synthetase family complex which enhances its ability to mediate an efficient antiviral response. The protein requires double-stranded RNA often produced during viral infections to achieve activation. This activation links the detection of viral components directly to antiviral actions effectively curtailing the spread of the virus within the host.
- Pathways
OAS3 participates in the interferon alpha/beta signaling pathway and the 2'-5'-oligoadenylate synthetase-RNase L pathway. These pathways are essential for mediating antiviral responses in innate immunity. In these pathways OAS3 interacts with related proteins such as RNase L and other members of the OAS family like OAS1 and OAS2 which collectively enhance the immune response against viral threats.
- Associated diseases and disorders
Alterations in OAS3 activity relate to diseases such as hepatitis and systemic lupus erythematosus (SLE). Abnormal OAS3 expression or function can affect host viral defense mechanisms contributing to chronic infection conditions like hepatitis. In SLE OAS3 alongside proteins like OAS1 and RNase L becomes relevant due to its role in inappropriately activating immune responses potentially leading to tissue damage. These insights into OAS3's role highlight its significance in both protective immunity and its potential involvement in autoimmunity.
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2 product images
Sanger Sequencing - Human OAS3 knockout A549 cell line (ab267212)
Allele-2: 1 bp deletion in exon 1.
Sanger Sequencing - Human OAS3 knockout A549 cell line (ab267212)
Allele-1: 5 bp deletion in exon1
Downloads
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