Human PCBD1 knockout HEK-293T cell line
- Advanced Validation
- What is this?
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- WB
Lab
Western blot - Human PCBD1 knockout HEK-293T cell line (AB266261)
Lanes 1-4 : Merged signal (red and green). Green - ab138518 observed at 12 kDa. Red - loading control ab8245 observed at 36 kDa.
ab138518 Anti-PCBD1 antibody [EPR9760(B)] was shown to specifically react with PCBD1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266261 (knockout cell lysate ab258095) was used. Wild-type and PCBD1 knockout samples were subjected to SDS-PAGE. ab138518 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4° at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PCBD1 antibody [EPR9760(B)] (<a href='/en-us/products/primary-antibodies/pcbd1-antibody-epr9760b-ab138518'>ab138518</a>) at 1/500 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
PCBD1 knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human PCBD1 knockout HEK-293T cell line (ab266261)
Lane 3:
Caco-2 cell lysate at 20 µg
Lane 4:
Human ovary cancer tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
false
- Cell Culture
Unknown
Cell Culture - Human PCBD1 knockout HEK-293T cell line (AB266261)
Representative images of PCBD1 knockout HEK293T cells, low and high confluency examples (top left and right respectively) and wild-type HEK293T cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using an EVOS M5000 microscope.
- Sanger seq
Unknown
Sanger Sequencing - Human PCBD1 knockout HEK-293T cell line (AB266261)
Homozygous : Insertion of the selection cassette in exon2
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Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PCBD1 interacts with tetrahydrobiopterin metabolism participating in neurotransmitter synthesis through its dehydratase function. It also acts as a dimerization cofactor for HNF1 (HNF1A) a transcription factor critical in gene regulation. The PCBD1 protein forms dimers or tetramers indicating its capability to participate in complex formation which enhances transcriptional regulation. This regulatory activity affects several genes involved in hepatic metabolism and development.
Pathways
PCBD1 primarily influences the tetrahydrobiopterin biosynthesis pathway and the regulation of gene expression pathway via HNF1A. By facilitating neurotransmitter production it indirectly plays a role in neurological pathways specifically those related to dopamine and serotonin synthesis. Through its transcriptional co-regulation with HNF1A PCBD1 impacts glucose metabolism and pancreatic functions linking it to metabolic pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com