JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB306749

Human PDE5A knockout U-87 MG cell line

Be the first to review this product! Submit a review

|

(0 Publication)

PDE5A KO cell line available to order. KO validated by. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.

Key facts

Cell type

U-87 MG

Species or organism

Human

Tissue

Brain

Form

Liquid

form

Disease

Glioblastoma

style
left-column

Product details

Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "2x1000000Cellsvial": { "sellingSize": "2 x 1000000 Cells/vial", "publicAssetCode":"ab306749-2x1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab306749 Human PDE5A knockout U-87 MG cell line", "number":"AB306749-CMP01" }, { "size":"1 x 1000000 Cells/vial", "name":"ab279979 Human wild-type U-87 MG cell line", "number":"AB306749-CMP02" } ] }, "1000000Cellsvial": { "sellingSize": "1000000 Cells/vial", "publicAssetCode":"ab306749-1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab306749 Human PDE5A knockout U-87 MG cell line", "number":"AB306749-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
PDE5A
Gene editing type
Knockout
Gene editing method
CRISPR technology
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C
style
left-column

Handling procedures

Initial handling guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium

EMEM + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE5A also known as PDE5 or phosphodiesterase type 5 plays a critical role in hydrolyzing cyclic guanosine monophosphate (cGMP) to its inactive form. This enzyme is part of the phosphodiesterase family and has a molecular mass of approximately 100 kDa. PDE5A is expressed mainly in the smooth muscle cells of blood vessels and in the corpus cavernosum of the penis. Other expression sites include the lung heart and skeletal muscle.
Biological function summary

PDE5A regulates intracellular levels of cGMP an important secondary messenger in various signaling pathways. It is not part of a large protein complex but directly influences the contractility of smooth muscle tissues. By controlling cGMP levels PDE5A modulates vascular tone and influences processes such as vasodilation. This regulation is essential for maintaining a balance in the contractile state of muscles impacting blood flow and other vital functions.

Pathways

PDE5A contributes significantly to the nitric oxide (NO)-cGMP signaling pathway which is important for vascular relaxation. In this pathway nitric oxide stimulates guanylate cyclase to produce cGMP which then activates specific kinases and ion channels. Related proteins such as guanylate cyclase and cGMP-dependent protein kinases work alongside PDE5A to mediate smooth muscle relaxation and vasodilation particularly in penile erectile tissue.

PDE5A shows strong connections to erectile dysfunction and pulmonary hypertension. In the case of erectile dysfunction impaired regulation of cGMP by PDE5A affects the relaxation of smooth muscle leading to inadequate blood flow. Pulmonary hypertension involves elevated blood pressure in the lung arteries which PDE5A influences through its role in controlling vascular tone. The enzyme guanylate cyclase associates closely with PDE5A in these contexts as both influence cGMP pathways involved in these conditions.
style
left-column
style
left-column

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

style
left-column

Product protocols

style
left-column

Target data

See full target information PDE5A
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB259945

Anti-PDE5A/PDE5 antibody [EPR24129-95]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody [EPR24129-95] (AB259945)

  • 0
  • 0
Proteins & Peptides

AB80330

Recombinant mouse PDE5A/PDE5 protein

Functional Studies - Recombinant mouse PDE5A/PDE5 protein (AB80330)

  • 0
  • 0
Biochemicals

AB141273

Cilostamide, PDE3 inhibitor

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com