Human PEG10 (EDR) knockout HeLa cell line
- Advanced Validation
- What is this?
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PEG10 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2.
View Alternative Names
AA407948, Edr, Embryonal carcinoma differentiation regulated, Embryonal carcinoma differentiation-regulated protein, HB 1, KIAA1051, MEF3 like 1, MEF3-like protein 1, MEF3L, MEF3L1, Mammalian retrotransposon-derived protein 2, Mar2, Mart2, MyEF 3, Myelin expression factor 3-like protein 1, PEG10 protein, PEG10_HUMAN, Paternally expressed 10, Paternally expressed gene 10 ORF1, Paternally expressed gene 10 protein, Putative uncharacterized protein PEG10, RGAG3, Retrotransposon gag domain containing 3, Retrotransposon gag domain-containing protein 3, Retrotransposon-derived gag-like polyprotein, Retrotransposon-derived protein PEG10, Ty3/Gypsy-like protein
- WB
Lab
Western blot - Human PEG10 (EDR) knockout HeLa cell line (AB265925)
Lanes 1-4 : Merged signal (red and green). Green - ab215035 observed at 100 kDa. Red - loading control ab7291 observed at 50 kDa.
ab215035 Anti-PEG10/EDR antibody [EPR20051] was shown to specifically react with PEG10/EDR in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265925 (knockout cell lysate ab258103) was used. Wild-type and PEG10/EDR knockout samples were subjected to SDS-PAGE. ab215035 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PEG10/EDR antibody [EPR20051] (<a href='/en-us/products/primary-antibodies/peg10-edr-antibody-epr20051-ab215035'>ab215035</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PEG10 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human PEG10 (EDR) knockout HeLa cell line (ab265925)
Lane 3:
HepG2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 80 kDa
Observed band size: 100 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PEG10 (EDR) knockout HeLa cell line (AB265925)
Homozygous : 1 bp deletion in exon 2.
- Sanger seq
Lab
Sanger Sequencing - Human PEG10 (EDR) knockout HeLa cell line (AB265925)
Sequencing chromatogram displaying sequence edit in exon 2
Reactivity data
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The PEG10 protein plays multiple roles especially in cell proliferation apoptosis inhibition and promoting cell survival. It can be part of larger protein complexes that modulate cellular responses to stress and developmental cues. The protein's multifaceted activities are important during embryogenesis where it supports trophoblast development and placental formation.
Pathways
PEG10 interacts with signaling cascades such as the TGF-beta signaling pathway and is involved in cell cycle regulation. PEG10 works alongside proteins like paternally expressed genes that connect with TGF-beta pathways influencing cellular differentiation and growth. Its integration in these pathways highlights its role in managing cellular environments and responses to cellular stress signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB215035
Anti-PEG10/EDR antibody [EPR20051]
RabMAb|Recombinant|KO Validated|Trial Size|Trial Size
![Western blot - Anti-PEG10/EDR antibody [EPR20051] (AB215035)](https://content.abcam.com/products/images/peg10-edr-antibody-epr20051-ab215035--western-blot-img21512.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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