PER1 KO cell line available to order. Free of charge wild type control provided.
A549
Human
Lung
Liquid
Circadian clock protein PERIOD 1, Circadian pacemaker protein Rigui, KIAA0482, MGC88021, PER1 protein, PER1_HUMAN, Period 1, Period circadian protein homolog 1, Period drosophila homolog of, Period homolog 1, RIGUI, hPER, hPER 1
PER1 KO cell line available to order. Free of charge wild type control provided.
A549
Human
Lung
Liquid
Carcinoma
PER1
Knockout
CRISPR technology
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
EU: 1 US: 1
Adherent
Male
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
F-12K + 10% FBS
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Dry Ice
-196°C
-196°C
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type A549 cell line (Human wild-type A549 cell line ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
This supplementary information is collated from multiple sources and compiled automatically.
The PER1 protein also known as Period Circadian Protein Homolog 1 forms part of the circadian rhythm machinery. It has a molecular weight of approximately 136 kDa. This protein is expressed in various tissues including brain liver and muscle cells. Mechanically PER1 modulates the oscillations in the expression of core circadian clock genes. By binding to DNA PER1 regulates transcription and sets circadian rhythms stabilizing the biological clock.
The PER1 protein plays a critical role in maintaining the body’s circadian cycles. It is part of a larger circadian clock complex that includes other components such as CLOCK and BMAL1. This protein ensures proper timing of sleep-wake cycles hormone release and other physiological processes. Although PER1 works with other proteins its individual expression influences the pace of the biological clock.
PER1 protein functions within the circadian rhythm pathway and impacts the regulation of the sleep-wake cycle. It interacts with the CLOCK and CRY proteins contributing to the transcription-translation feedback loop fundamental for circadian timing. This complex interplay helps maintain synchronization with environmental light-dark cycles affecting various biological processes.
Abnormalities in PER1 expression associate with sleep disorders like familial advanced sleep phase syndrome (FASPS) and certain mood disorders. Disruptions in its regulatory network involving proteins like PER2 can lead to misalignments in circadian rhythms. These changes can result in issues with sleep patterns and contribute to mood regulation problems.
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