Human PI4K2B knockout HeLa cell line
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PI4K2B KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
View Alternative Names
2610042N09Rik, 4933409G22Rik, FLJ11105, P4K2B_HUMAN, PI4K 2 beta, PI4K IIb, PI4KII-BETA, PIK42B, Phosphatidylinositol 4-kinase type 2-beta, Phosphatidylinositol 4-kinase type II-beta, Type II phosphatidylinositol 4 kinase beta isoform
- Sanger seq
Unknown
Sanger Sequencing - Human PI4K2B knockout HeLa cell line (AB264683)
Homozygous : 1 bp insertion in exon 1.
Product details
Recommended control: Human wild-type HeLa cell line (ab255448). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PI4K2B influences multiple cellular functions particularly those related to membrane trafficking and cell signaling. Although not part of a larger known complex PI4K2B's enzymatic production of PI4P has many downstream effects impacting endosomal dynamics and vesicular trafficking. The protein's involvement in membrane dynamics suggests important roles in regulating cell movement and recycling of membrane components affecting how cells respond to their surroundings.
Pathways
PI4K2B integrates into the broader phosphoinositide pathway that regulates cellular communication and processes. It closely interacts with proteins like AKT and RAB proteins which are essential in the regulation of vesicle transport and signal transduction. The production of PI4P by PI4K2B contributes to the modulation of the PI3K/AKT signaling pathway impacting cellular growth survival and metabolism. This pathway's complexity demonstrates PI4K2B's role in coordinating multiple biological reactions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com