PIWIL4 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided.
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Key facts
- Cell type
- MCF7
- Species or organism
- Human
- Tissue
- Breast
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
Alternative names
DKFZp686P01248, FLJ36156, HILI 2, HIWI 2, Miwi 2 protein, Miwi2, PIWI like protein, PIWIL 4, PIWL4_HUMAN, Piwi like 2, Piwi like 4, Piwi like 4 (Drosophila), Piwi like RNA mediated gene silencing 4, Piwi-like protein 4
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PIWIL4 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided.
Key facts
- Cell type
- MCF7
- Species or organism
- Human
- Tissue
- Breast
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- PIWIL4
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- Slow to trypsinise.
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 5-7x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- MEM + 10% FBS + 0.01 mg/ml bovine insulin
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
Recommended control: Human wild-type MCF7 cell line (ab288560). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PIWIL4 also known as PIWI-like 4 is a protein involved in RNA-mediated gene silencing with a molecular weight of approximately 97 kDa. It is expressed mostly in germ cell tissues including the testes and ovaries. PIWIL4 belongs to the PIWI subfamily of Argonaute proteins which is critical for the regulation of gene expression at the transcriptional and post-transcriptional levels.
- Biological function summary
PIWIL4 plays an important role in the process of germ cell development and maintenance. It is essential for the formation of piRNA clusters and interacts with small non-coding RNA known as PIWI-interacting RNA (piRNA). It functions as part of a protein complex that facilitates the silencing of transposable elements and the regulation of gene expression therefore safeguarding genome integrity in germ cells.
- Pathways
PIWIL4 integrates within the piRNA biogenesis pathway and RNA interference (RNAi) pathway key processes in cellular defense mechanisms. It associates with other PIWI family members such as PIWIL1 and PIWIL2 which collaborate to cleave and silence transposable elements and undesirable genetic elements. This function is critical in pausing transposition events that can disrupt genomic stability.
- Associated diseases and disorders
PIWIL4 has connections to certain cancers such as testicular germ cell tumor due to its role in regulating cell proliferation and genome protection. Abnormal expression or malfunction of PIWIL4 has implications in the development of infertility as it influences germ cell maturation. Investigations also assess its interactions with proteins like MAEL and TDRD1 which contribute to the maintenance of spermatogenic functions in testicular tissues.
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1 product image
Next Generation Sequencing - Human PIWIL4 knockout MCF7 cell line (ab289393)
50 bp deletion in exon 4, CCDS31656.1
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