Human PIWIL4 knockout MCF7 cell line
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PIWIL4 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
DKFZp686P01248, FLJ36156, HILI 2, HIWI 2, Miwi 2 protein, Miwi2, PIWI like protein, PIWIL 4, PIWL4_HUMAN, Piwi like 2, Piwi like 4, Piwi like 4 (Drosophila), Piwi like RNA mediated gene silencing 4, Piwi-like protein 4
- NGS
Lab
Next Generation Sequencing - Human PIWIL4 knockout MCF7 cell line (AB289393)
50 bp deletion in exon 4, CCDS31656.1
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- Slow to trypsinise.
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 5-7x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
MEM + 10% FBS + 0.01 mg/ml bovine insulin
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PIWIL4 plays an important role in the process of germ cell development and maintenance. It is essential for the formation of piRNA clusters and interacts with small non-coding RNA known as PIWI-interacting RNA (piRNA). It functions as part of a protein complex that facilitates the silencing of transposable elements and the regulation of gene expression therefore safeguarding genome integrity in germ cells.
Pathways
PIWIL4 integrates within the piRNA biogenesis pathway and RNA interference (RNAi) pathway key processes in cellular defense mechanisms. It associates with other PIWI family members such as PIWIL1 and PIWIL2 which collaborate to cleave and silence transposable elements and undesirable genetic elements. This function is critical in pausing transposition events that can disrupt genomic stability.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com