Human PLXNB2 (Plexin B2/MM1) knockout HEK-293 cell line
- Advanced Validation
- What is this?
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(0 Publication)
- WB
Supplier Data
Western blot - Human PLXNB2 (Plexin B2/MM1) knockout HEK-293 cell line (AB269468)
Lanes 1 - 4 : Merged signal (red and green). Green - ab193355 observed at 80 kDa. Red - loading control, ab7291, observed at 50 kDa.
ab193355 was shown to specifically react with in wild-type HEK-293 cells as signal was lost in PLXNB2 knockout cell line ab269468 (knockout cell lysate ab269633). Wild-type and PLXNB2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab193355 and ab7291 (Mouse anti-Tubulin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (<a href='/en-us/products/primary-antibodies/plexin-b2-mm1-antibody-epr9965-ab193355'>ab193355</a>) at 1/5000 dilution
Lane 1:
Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
PLXNB2 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
Western blot - Human PLXNB2 (Plexin B2/MM1) knockout HEK-293 cell line (ab269468)
Lane 3:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4:
Human brain whole cell lysate at 20 µg
Predicted band size: 204 kDa
Observed band size: 80 kDa
false
- NGS
Supplier Data
Next Generation Sequencing - Human PLXNB2 (Plexin B2/MM1) knockout HEK-293 cell line (AB269468)
1 bp insertion (allele 1) and 5 bp deletion (allele 2) after Arg441 of the WT protein
Complementary Products
Primary Antibodies
AB193355
Anti-Plexin B2/MM1 antibody [EPR9965]
RabMAb|Recombinant|KO Validated
![Flow Cytometry (Intracellular) - Anti-Plexin B2/MM1 antibody [EPR9965] (AB193355)](https://content.abcam.com/products/images/plexin-b2-mm1-antibody-epr9965-ab193355--flow-cytometry-intracellular-img106370.jpg)
- 3
- 1
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Plexin B2 plays a role in cellular signaling processes that are essential for neural patterning and tissue morphogenesis. It binds with semaphorins particularly Sema4C to guide axonal repulsion in neural development. Plexin B2 forms part of a receptor complex that helps cells interpret external semaphorin signals influencing cell movement and positioning. These interactions contribute to the remodeling of neural circuits which is important during both prenatal development and adult neural function.
Pathways
Plexin B2 participates in pathways engaging Rho GTPases which are important for cytoskeletal organization and cell movement. Plexin B2 influences the RhoA and Rac1 pathways impacting actin cytoskeleton dynamics and cell adhesion processes. These pathways where Plexin B2 acts with other Rho family proteins help transmit the changes induced by semaphorin binding altering cellular orientation and migration.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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