Human POLQ knockout A549 cell line
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POLQ KO cell line available to order. KO validated by. Free of charge wild type control provided.
View Alternative Names
DKFZp781A0112, DNA polymerase eta, DNA polymerase theta, DPOLQ_HUMAN, POLQ, PRO0327, Polymerase (DNA directed) theta
- Sanger seq
Supplier Data
Sanger Sequencing - Human POLQ knockout A549 cell line (AB286655)
105 bp deletion in Exon 1 and Intron 1 (uppercase : Exon, lowercase : Intron)
Reactivity data
Product details
Recommended control: Human wild-type A549 cell line (ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
Culture medium
F-12K + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DNA repair processes depend heavily on DNA Polymerase theta. This enzyme participates in a distinctive mechanism known as microhomology-mediated end-joining (MMEJ) which repairs double-strand breaks in DNA. Unlike canonical end-joining pathways MMEJ employs short homologous sequences to mediate repair where Pol θ serves a critical role. Although Pol θ acts independently it can still interact with other repair factors to ensure efficient DNA end-joining highlighting its importance.
Pathways
Maintenance of genome stability relies on pathways involving DNA Polymerase theta. The enzyme is an important component of the MMEJ pathway an alternative mechanism to the more common non-homologous end-joining (NHEJ). In this capacity Pol θ cooperates with proteins involved in DNA damage response such as BRCA1 and BRCA2 which help facilitate repair processes and support cell survival under genotoxic stress conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com