Human PPP1R2 (Protein phosphatase 1 inhibitor subunit 2) knockout HeLa cell line
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- Sanger seq
Unknown
Sanger Sequencing - Human PPP1R2 (Protein phosphatase 1 inhibitor subunit 2) knockout HeLa cell line (AB265684)
Homozygous : Insertion of the selection cassette in exon 1.
Complementary Products
Primary Antibodies
AB27850
Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody
- 0
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Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PPP1R2 plays a critical role in cellular regulation by modulating PP1 activity. It forms a complex with PP1 in which PPP1R2 binds directly to the catalytic subunit of PP1 inhibiting its phosphatase activity. By controlling PP1 activity PPP1R2 impacts processes like muscle contraction protein synthesis and neuronal signaling making it an active participant in maintaining cellular homeostasis.
Pathways
PPP1R2 functions within important signaling and dephosphorylation pathways. It is notably involved in the glycogen metabolism pathway where it regulates PP1 activity influencing glycogen synthesis and breakdown. Additionally it participates in the cell cycle regulation pathway interacting with other proteins such as PP1 to control cell proliferation and division. The regulation of these pathways by PPP1R2 highlights its importance in key cellular activities.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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