PPP4R2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 1 bp insertion in exon 1.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 1 bp insertion in exon 1
Alternative names
BE691708, C230060M08Rik, MGC131930, PP4R2_HUMAN, Serine/threonine-protein phosphatase 4 regulatory subunit 2, protein phosphatase 4, regulatory subunit 2
Recommended products
PPP4R2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 1 bp insertion in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 1 bp insertion in exon 1
- Concentration
Properties
- Gene name
- PPP4R2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein phosphatase 4 regulatory subunit 2 commonly known as PPP4R2 serves as a regulatory subunit in the protein phosphatase 4 (PP4) complex. It has an approximate mass of 50 kDa. PPP4R2 is mainly expressed in the cytoplasm of cells where it regulates the activity and specificity of PP4. It is involved in various cellular processes due to its capacity to modulate PP4's activity.
- Biological function summary
PPP4R2 plays a significant role in DNA damage repair processes. PPP4R2 is part of the PP4 complex and together with other components it oversees dephosphorylation processes post DNA damage which is essential for cell cycle progression and genomic stability. It collaborates with other proteins in the complex to ensure accurate DNA repair maintaining proper cellular function and preventing mutations.
- Pathways
PPP4R2 is notably involved in the DNA repair pathway and cell cycle regulation pathway. These pathways are critical for maintaining cell integrity and viability. Within the DNA repair pathway PPP4R2 operates closely with proteins like PP4C the catalytic subunit of PP4 which performs dephosphorylation tasks essential for DNA damage response. In the cell cycle regulation pathway PPP4R2 ensures appropriate cell cycle checkpoints by interacting with multiple cellular proteins which helps in maintaining genomic stability.
- Associated diseases and disorders
Research links PPP4R2 to cancer and neurodegenerative diseases highlighting its importance in maintaining genomic integrity. In cancer alterations in PPP4R2 expression or function can affect DNA repair leading to genomic instability and tumorigenesis. PPP4R2 interacts with proteins such as CHK2 that are important in the DNA damage response. Moreover abnormal PPP4R2 activity impacts neurodegenerative conditions where defects in DNA repair mechanisms are implicated. Understanding its role in these contexts aids in potential therapeutic targeting strategies.
Product promise
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2 product images
Sanger Sequencing - Human PPP4R2 knockout HEK-293T cell line (ab266206)
Allele-1: 10 bp deletion in exon 1
Sanger Sequencing - Human PPP4R2 knockout HEK-293T cell line (ab266206)
Allele-2: 1 bp insertion in exon 1.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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