AB266842

Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line

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PRDX1 KO cell line available to order. KO validated by Immunocytochemistry, Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.

View Alternative Names

PRDX1, TDPX2, PAGA, Proliferation-associated gene protein, Thioredoxin-dependent peroxide reductase 2, Thioredoxin-dependent peroxiredoxin 1, PAG, Natural killer cell-enhancing factor A, Peroxiredoxin-1, NKEF-A, PAGB, Thioredoxin peroxidase 2

5 Images

Immunocytochemistry/ Immunofluorescence - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (AB266842)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (AB266842)

Peroxiredoxin 1/PAG (PRDX1) staining observed in wild-type HEK293T cells and PRDX1 knockout HEK293T cells (ab266842). The cells were fixed with 100% methanol then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab109506 at 1/50 dilution and followed by secondary antibody ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (shown in green). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain at 1/200 dilution (shown in red). Nuclear DNA was labelled in blue with DAPI.

Confocal image showing cytoplasmic staining in wild-type HEK-293Tcell line, and no staining in PRDX1 knockout HEK-293T cell line.

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Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (AB266842)

Lanes 1-3 : Merged signal (red and green). Green - ab109506 observed at 26 kDa. Red - loading control ab8245 observed at 36 kDa.

ab109506 Anti-Peroxiredoxin 1/PAG antibody [EPR5434] was shown to specifically react with Peroxiredoxin 1/PAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266842 (knockout cell lysate ab257040) was used. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab109506 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4° at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5434] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-1-pag-antibody-epr5434-ab109506'>ab109506</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

PRDX1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (ab266842)

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 22 kDa

Observed band size: 26 kDa

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Lab

Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (AB266842)

Lanes 1-3 : Merged signal (red and green). Green - ab109498 observed at 26 kDa. Red - loading control ab8245 observed at 36 kDa.

ab109498 Anti-Peroxiredoxin 1/PAG antibody [EPR5433] was shown to specifically react with Peroxiredoxin 1/PAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266842 (knockout cell lysate ab257040) was used. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab109498 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-1-pag-antibody-epr5433-ab109498'>ab109498</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

PRDX1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (ab266842)

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Predicted band size: 22 kDa,41 kDa,45 kDa,78 kDa

Observed band size: 25 kDa,26 kDa,47 kDa,49 kDa,78 kDa

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Cell Culture - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (AB266842)

Cell Culture

Unknown

Cell Culture - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (AB266842)

Representative images of PRDX1 knockout HEK293T cells, low and high confluency examples (top left and right respectively) and wild-type HEK293T cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using an EVOS M5000 microscope.

Sanger Sequencing - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (AB266842)

Sanger seq

Unknown

Sanger Sequencing - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (AB266842)

Homozygous : 1 bp deletion in exon2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Form

Liquid

form

Knockout validation

Immunocytochemistry,Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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Product details

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name

PRDX1

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Immunocytochemistry, Sanger Sequencing, Western blot

Zygosity

Homozygous

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-196°C

Appropriate long-term storage conditions

-196°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peroxiredoxin 1 also known as PAG plays an important role as an antioxidant enzyme. It is part of the peroxiredoxin family with a molecular mass of around 22 kDa. This protein helps reduce hydrogen peroxide and organic hydroperoxides to water and corresponding alcohols using reducing equivalents provided by thiol-containing donor molecules. Peroxiredoxin 1/PAG expresses widely in human tissues and cells including liver and erythrocytes reflecting its involvement in cellular redox regulation.

Biological function summary

Peroxiredoxin 1 helps maintain cellular homeostasis by protecting cells from oxidative stress. It forms homodimers or interacts with other proteins to propagate cellular responses to changes in redox state. As an important member of the antioxidant defense mechanism it contributes to cellular signaling through modulation of hydrogen peroxide signaling pathways. The protein also plays a role in cell proliferation and apoptosis making it significant for cellular health and disease prevention.

Pathways

Peroxiredoxin 1 participates in critical processes such as the MAPK signaling and TGF-beta pathways. It interacts with MAPK-related proteins influencing cell survival and growth. Peroxiredoxin 1 indirectly modulates downstream signaling molecules that contribute to cellular responses. In these pathways the protein's antioxidant properties allow regulation of reactive oxygen species levels which directly affect cellular signaling cascades.

Peroxiredoxin 1 is relevant to cancer and neurodegenerative disorders. Overexpression or misregulation of this protein has been linked to various cancers where it potentially interacts with oncogenic proteins like c-Myc to promote tumor progression. Additionally abnormal levels of Peroxiredoxin 1 associate with neurodegenerative diseases as oxidative stress is a common factor in their pathology. These connections suggest the protein as a promising target in therapeutic interventions for these diseases.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. 1. Thaw the vial in 37°C water bath for approximately 1-2 minutes. 2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution. 3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25. 4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed. 5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines

All seeding densities should be based on cell counts gained by established methods.
A guide seeding density of 2x10⁴ cells/cm² is recommended.
Cells should be passaged when they have achieved 80-90% confluence.

Culture medium

DMEM (High Glucose) + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information PRDX1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 16:1599691 PubMed40693141

2025

Tumor-associated bacteria activate PRDX1-driven glycolysis to promote immune evasion and PD-1 antibody resistance in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhang,Xi Lan,Liquan Cai,Xunfeng Gao,Feng Gao,Dan Yu,Jinlong Zhang,Jinhui Zhang,Qinwen Tai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line

Immunocytochemistry/ Immunofluorescence - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (AB266842)

Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (AB266842)

Western blot - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (AB266842)

Cell Culture - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (AB266842)

Sanger Sequencing - Human PRDX1 (Peroxiredoxin 1/PAG) knockout HEK-293T cell line (AB266842)

Key facts

Cell type

Species or organism

Tissue

Form

Knockout validation

Mutation description

Complementary Products

Cell Lines & Lysates

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

What's included?

Properties and storage information

Gene name

Gene editing type

Gene editing method

Knockout validation

Zygosity

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Quality control

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

Initial handling guidelines

Subculture guidelines

Culture medium

Cryopreservation medium

Product protocols

Target data

Publications (1)

Product promise