Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line
- Advanced Validation
- What is this?
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PRDX2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
Epididymis secretory sperm binding protein Li 2a, HEL S 2a, MGC4104, NKEF-B, Natural Killer Enhancing Factor B, Natural killer cell-enhancing factor B, PRDX2_HUMAN, PRX2, PRXII, PTX 1, Peroxiredoxin-2, PrP, TDPX1, TPX1, TSA, Thiol Specific Antioxidant 1, Thiol-specific antioxidant protein, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, Torin
- WB
Lab
Western blot - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line (AB266392)
Lanes 1- 2 : Merged signal (red and green). Green - ab109367 observed at 22 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab109367 was shown to react with Peroxiredoxin 2/PRP in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266392 (knockout cell lysate ab257041) was used. Wild-type HEK-293T and PRDX2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109367 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4° at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-2-prp-antibody-epr5154-ab109367'>ab109367</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
PRDX2 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line (ab266392)
Predicted band size: 22 kDa
Observed band size: 22 kDa
false
- WB
Lab
Western blot - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line (AB266392)
Lanes 1- 2 : Merged signal (red and green). Green - ab133481 observed at 22 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab133481 was shown to react with Peroxiredoxin 2/PRP in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266392 (knockout cell lysate ab257041) was used. Wild-type HEK-293T and PRDX2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133481 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4° at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (<a href='/en-us/products/primary-antibodies/peroxiredoxin-2-prp-antibody-epr5155-ab133481'>ab133481</a>) at 1/5000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
PRDX2 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line (ab266392)
Predicted band size: 22 kDa
Observed band size: 22 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PRDX2 (Peroxiredoxin 2/PRP) knockout HEK-293T cell line (AB266392)
Homozygous : 2 bp deletion in exon 2
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Peroxiredoxins like PRDX2 function by breaking down hydrogen peroxide and organic hydroperoxides safeguarding cells from oxidative harm. PRDX2 often forms homodimers or higher-order oligomers which influence its catalytic efficiency and chaperone activity. Within the cell it not only acts independently but also associates with other cellular components reflecting its participation in important cellular processes including cell proliferation and apoptosis.
Pathways
PRDX2 engages significantly within antioxidant defense systems specifically the thioredoxin pathway. It interacts with thioredoxin reductase and thioredoxin to facilitate the reduction of peroxides maintaining the cell's redox balance. Furthermore PRDX2 intersects with cellular signaling pathways associated with inflammation and the regulation of cell death where proteins like NRF2 and KEAP1 play important roles in managing oxidant-inducible gene expression.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB109367
Anti-Peroxiredoxin 2/PRP antibody [EPR5154]
RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (AB109367)](https://content.abcam.com/products/images/peroxiredoxin-2-prp-antibody-epr5154-ab109367--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84884.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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