Human PRKCD knockout U-87 MG cell line

• WB

Lab

Western blot - Human PRKCD knockout U-87 MG cell line (AB306756)

Western blot : Anti-PRKCD antibody [14/PKC delta] (ab307233) staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab307233 was shown to bind specifically to PRKCD. A band was observed at 75 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in PRKCD knockout cell line. To generate this image, wild-type and PRKCD knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-PKC delta antibody [14/PKC delta] (<a href='/en-us/products/primary-antibodies/pkc-delta-antibody-14-pkc-delta-ab307233'>ab307233</a>) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG cell lysate at 20 µg

Lane 2:

Western blot - Human PRKCD knockout U-87 MG cell line (ab306756)

Lane 2:

PRKCD knockout U-87 MG cell lysate at 20 µg

Secondary

Lanes 1 - 2:

Goat anti-Mouse IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 2:

Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Observed band size: 75 kDa

false

• WB

Lab

Western blot - Human PRKCD knockout U-87 MG cell line (AB306756)

Western blot : Anti-PRKCD antibody [EPR17075] (ab182126) staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab182126 was shown to bind specifically to PRKCD. A band was observed at 75-80 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in PRKCD knockout cell line. To generate this image, wild-type and PRKCD knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-PKC delta antibody [EPR17075] (<a href='/en-us/products/primary-antibodies/pkc-delta-antibody-epr17075-ab182126'>ab182126</a>) at 1/5000 dilution

Lane 1:

Wild-type U-87 MG cell lysate at 20 µg

Lane 2:

Western blot - Human PRKCD knockout U-87 MG cell line (ab306756)

Lane 2:

PRKCD knockout U-87 MG cell lysate at 20 µg

Secondary

Lanes 1 - 2:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 2:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 75-80 kDa

false

• NGS

Lab

Next Generation Sequencing - Human PRKCD knockout U-87 MG cell line (AB306756)

83 bp deletion (allele 1), 13 bp deletion (allele 2) in exon 4, CCDS2870.1