Human PSMD8 knockout HEK-293T cell line
Be the first to review this product! Submit a review
|
(0 Publication)
PSMD8 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 1 and 22 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
26S proteasome non-ATPase regulatory subunit 8, 26S proteasome regulatory subunit RPN12, 26S proteasome regulatory subunit S14, 26S proteasome regulatory subunit p31, AAV38494, HIP6, HYPF, MGC1660, Nin1p, P31, PSMD8_HUMAN, Proteasome (prosome macropain) 26S subunit non ATPase 8, Rpn12
- Sanger seq
Unknown
Sanger Sequencing - Human PSMD8 knockout HEK-293T cell line (AB266329)
Allele-1 : 22 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human PSMD8 knockout HEK-293T cell line (AB266329)
Allele-2 : 19 bp deletion in exon 1.
Product details
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PSMD8 contributes to the assembly and function of the 26S proteasome which is a multi-subunit complex responsible for degrading ubiquitinated proteins. It acts as a part of the regulatory particle enabling substrate recognition and unfolding. Through its function PSMD8 is involved in the ubiquitin-proteasome system which is key in cellular regulation mechanisms such as cell cycle control apoptosis and DNA repair. This highlights its importance in maintaining cell function and integrity.
Pathways
Protein homeostasis and degradation represent important pathways in which PSMD8 participates. It plays a role in the ubiquitin-proteasome pathway interacting with proteins within this system like Rpn11 and Rpt5 which are also parts of the proteasome complex. Additionally the involvement of PSMD8 in the protein degradation pathway links it to the regulation of transcription factors influencing various signaling pathways that control cell proliferation and differentiation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com