PTGER4 KO cell line available to order. Free of charge wild type control provided.
PC-3
Human
Prostate
Liquid
EP 4, EP4R, MGC126583, PE2R4_HUMAN, PGE receptor EP4 subtype, PGE2 receptor EP4 subtype, PTGER 2, PTGER 4, Prostaglandin E receptor 4, Prostaglandin E receptor 4 subtype EP4, Prostaglandin E2 receptor, Prostaglandin E2 receptor EP4 subtype, Prostanoid EP4 receptor
PTGER4 KO cell line available to order. Free of charge wild type control provided.
PC-3
Human
Prostate
Liquid
Adenocarcinoma
PTGER4
Knockout
CRISPR technology
EU: 1 US: 1
Adherent
Male
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
F-12K + 10% FBS
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Dry Ice
-196°C
-196°C
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type PC-3 cell line (Human wild-type PC-3 cell line ab290718). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
This supplementary information is collated from multiple sources and compiled automatically.
The PGE2 receptor EP4 subtype also known as PTGER4 is a member of the G protein-coupled receptor (GPCR) family. This receptor binds prostaglandin E2 and plays a role in various physiological processes. The protein has an approximate mass of 65 kDa. EP4 receptor is widely expressed in tissues such as the immune system bone gastrointestinal tract and reproductive organs indicating its diverse functional implications. Additionally EP4 receptor is activated by PGE2 EP4 agonists which help in modulating its activity.
EP4 receptor influences numerous functions including inflammation immune response and bone metabolism. The receptor is not part of a larger complex but interacts with different signaling molecules and secondary messengers during its activity. It modulates responses mainly through cAMP which further amplifies or reduces cellular responses depending on the context. These biological roles display the versatile effects EP4 can exert during various physiological scenarios.
The EP4 receptor is a significant component in the prostaglandin signaling pathway. This pathway involves the conversion of arachidonic acid to prostaglandin E2 followed by binding to EP4 triggering downstream effects via cAMP-related signals. Within this pathway it interacts closely with proteins like adenylate cyclase which plays a role in regulating cAMP levels. EP4 also participates in the Wnt signaling pathway where it can either enhance or inhibit processes by interacting with other signaling cascade proteins.
EP4 receptor has connections with conditions like rheumatoid arthritis and bone-related diseases. In rheumatoid arthritis excessive signaling through EP4 exacerbates inflammatory responses in joint tissue. The receptor potentially associates with inflammatory cytokines like tumor necrosis factor-alpha (TNF-alpha) contributing to disease severity. In bone-related diseases such as osteoporosis EP4 influences bone remodeling and homeostasis linking to proteins involved in bone formation and resorption dynamics.
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