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AB270477

Human PTGS1 knockout A-431 cell line

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PTGS1 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9; X = 28 deletion, 2 deletion, 1 deletion; Frameshift: 99.86%. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.

View Alternative Names

Cyclooxygenase 3, included, Cyclooxygenase-1, EC 1.14.99.1, PCOX1, PGG/HS, PGH synthase 1, PGH1_HUMAN, PGHS-1, PHS 1, PTGHS, PTGS1, Partial COX1 proteins, included, Prostaglandin G/H synthase 1, Prostaglandin H2 synthase 1, Prostaglandin-endoperoxide synthase 1, Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)

3 Images
Western blot - Human PTGS1 knockout A-431 cell line (AB270477)
  • WB

Lab

Western blot - Human PTGS1 knockout A-431 cell line (AB270477)

Lanes 1 - 4:

Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (<a href='/en-us/products/primary-antibodies/cox1-cyclooxygenase-1-antibody-epr5867-ab133319'>ab133319</a>) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/cox1-cyclooxygenase-1-antibody-epr5867-bsa-and-azide-free-ab248471'>ab248471</a>) at 1/1000 dilution

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

Western blot - Human PTGS1 knockout A-431 cell line (ab270477) at 20 µg

Lane 2:

Western blot - Human PTGS1 knockout A-431 cell lysate (ab270500) at 20 µg

Lane 2:

PTGS1 knockout A431 cell lysate at 20 µg

Lane 3:

C2C12 cell lysate at 20 µg

Lane 4:

MOLT-4 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 70 kDa

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Western blot - Human PTGS1 knockout A-431 cell line (AB270477)
  • WB

Lab

Western blot - Human PTGS1 knockout A-431 cell line (AB270477)

Lanes 1 - 4:

Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/cox1-cyclooxygenase-1-antibody-epr5866-bsa-and-azide-free-ab219375'>ab219375</a>) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (<a href='/en-us/products/primary-antibodies/cox1-cyclooxygenase-1-antibody-epr5866-ab109025'>ab109025</a>) at 1/1000 dilution

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

Western blot - Human PTGS1 knockout A-431 cell lysate (ab270500)

Lane 2:

PTGS1 knockout A431 cell lysate at 20 µg

Lane 2:

Western blot - Human PTGS1 knockout A-431 cell line (ab270477)

Lane 3:

C2C12 cell lysate at 20 µg

Lane 4:

MOLT-4 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 70 kDa

false

Next Generation Sequencing - Human PTGS1 knockout A-431 cell line (AB270477)
  • NGS

Supplier Data

Next Generation Sequencing - Human PTGS1 knockout A-431 cell line (AB270477)

Knockout achieved by CRISPR/Cas9; X = 28 bp deletion, 2 bp deletion, 1 bp deletion; Frameshift = 99.6%

Key facts

Cell type

A-431

Species or organism

Human

Tissue

Skin

Form

Liquid

form

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9; X = 28 deletion, 2 deletion, 1 deletion; Frameshift: 99.86%

Disease

Epidermoid Carcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "NGS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PTGS1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Dry Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
-196°C|-80°C
Appropriate long-term storage conditions
-196°C
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Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium

DMEM (High Glucose) + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cyclooxygenase 1 (COX-1) also known as COX1 cyclooxygenase-1 COX one and the COX1 protein is an important enzyme in the conversion of arachidonic acid to prostaglandins. It is a heme-containing dimer enzyme with a molecular weight of approximately 70 kDa. COX-1 is widely expressed in most tissues and cell types where it plays a significant role in maintaining homeostatic functions. This protein is constitutively active meaning it is often active under normal physiological conditions.
Biological function summary

COX-1 is involved in producing prostaglandins that regulate a variety of normal physiological processes including gastric mucosal protection renal blood flow and platelet aggregation. Unlike COX-2 COX-1 is not induced by inflammatory stimuli and is not part of an inducible complex. It serves to maintain essential physiological functions in various organs and systems making its activity critical for cellular maintenance.

Pathways

COX-1 is primarily involved in the prostaglandin biosynthesis pathway. It converts arachidonic acid into prostaglandin H2 a precursor for other prostaglandins and thromboxanes. Thromboxane A2 produced from this pathway plays an important role in platelet aggregation and vasoconstriction linking COX-1's functions with hemostatic processes. Another protein involved in this pathway is thromboxane synthase which further processes the products of COX-1 activity.

COX-1's role connects it closely to conditions like peptic ulcers and cardiovascular diseases. Inhibition of COX-1 by nonsteroidal anti-inflammatory drugs (NSAIDs) can lead to gastric mucosal damage contributing to the development of peptic ulcers. Additionally due to its involvement in platelet aggregation COX-1 affects thrombotic diseases. COX-1's interactions with proteins such as COX-2 become relevant in inflammation and pain management where selective inhibition of COX-2 is sought to reduce adverse effects related to COX-1.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Target data

See full target information PTGS1
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Product promise

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