PTGS1 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9; X = 28 deletion, 2 deletion, 1 deletion; Frameshift: 99.86%.
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Key facts
- Cell type
- A-431
- Species or organism
- Human
- Tissue
- Skin
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing, Western blot
- Mutation description
- Knockout achieved by CRISPR/Cas9; X = 28 deletion, 2 deletion, 1 deletion; Frameshift: 99.86%
Alternative names
Cyclooxygenase 3, included, Cyclooxygenase-1, EC 1.14.99.1, PCOX1, PGG/HS, PGH synthase 1, PGH1_HUMAN, PGHS-1, PHS 1, PTGHS, PTGS1, Partial COX1 proteins, included, Prostaglandin G/H synthase 1, Prostaglandin H2 synthase 1, Prostaglandin-endoperoxide synthase 1, Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
Recommended products
PTGS1 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9; X = 28 deletion, 2 deletion, 1 deletion; Frameshift: 99.86%.
Key facts
- Cell type
- A-431
- Species or organism
- Human
- Tissue
- Skin
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing, Western blot
- Mutation description
- Knockout achieved by CRISPR/Cas9; X = 28 deletion, 2 deletion, 1 deletion; Frameshift: 99.86%
- Disease
- Epidermoid Carcinoma
- Concentration
Properties
- Gene name
- PTGS1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- -196°C, -80°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cyclooxygenase 1 (COX-1) also known as COX1 cyclooxygenase-1 COX one and the COX1 protein is an important enzyme in the conversion of arachidonic acid to prostaglandins. It is a heme-containing dimer enzyme with a molecular weight of approximately 70 kDa. COX-1 is widely expressed in most tissues and cell types where it plays a significant role in maintaining homeostatic functions. This protein is constitutively active meaning it is often active under normal physiological conditions.
- Biological function summary
COX-1 is involved in producing prostaglandins that regulate a variety of normal physiological processes including gastric mucosal protection renal blood flow and platelet aggregation. Unlike COX-2 COX-1 is not induced by inflammatory stimuli and is not part of an inducible complex. It serves to maintain essential physiological functions in various organs and systems making its activity critical for cellular maintenance.
- Pathways
COX-1 is primarily involved in the prostaglandin biosynthesis pathway. It converts arachidonic acid into prostaglandin H2 a precursor for other prostaglandins and thromboxanes. Thromboxane A2 produced from this pathway plays an important role in platelet aggregation and vasoconstriction linking COX-1's functions with hemostatic processes. Another protein involved in this pathway is thromboxane synthase which further processes the products of COX-1 activity.
- Associated diseases and disorders
COX-1’s role connects it closely to conditions like peptic ulcers and cardiovascular diseases. Inhibition of COX-1 by nonsteroidal anti-inflammatory drugs (NSAIDs) can lead to gastric mucosal damage contributing to the development of peptic ulcers. Additionally due to its involvement in platelet aggregation COX-1 affects thrombotic diseases. COX-1's interactions with proteins such as COX-2 become relevant in inflammation and pain management where selective inhibition of COX-2 is sought to reduce adverse effects related to COX-1.
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3 product images
Next Generation Sequencing - Human PTGS1 knockout A-431 cell line (ab270477)
Knockout achieved by CRISPR/Cas9; X = 28 bp deletion, 2 bp deletion, 1 bp deletion; Frameshift = 99.6%
Western blot - Human PTGS1 knockout A-431 cell line (ab270477)
Lanes 1 - 4: Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] ab133319) at 1/1000 dilution
Lanes 1 - 4: Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] - BSA and Azide free (Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] - BSA and Azide free ab248471) at 1/1000 dilution
Lane 1: Wild-type A431 cell lysate at 20 µg
Lane 2: Western blot - Human PTGS1 knockout A-431 cell line (ab270477) at 20 µg
Lane 2: Western blot - Human PTGS1 knockout A-431 cell lysate (ab270500) at 20 µg
Lane 2: PTGS1 knockout A431 cell lysate at 20 µg
Lane 3: C2C12 cell lysate at 20 µg
Lane 4: MOLT-4 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 70 kDa
Western blot - Human PTGS1 knockout A-431 cell line (ab270477)
Lanes 1 - 4: Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] - BSA and Azide free (Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] - BSA and Azide free ab219375) at 1/1000 dilution
Lanes 1 - 4: Western blot - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] ab109025) at 1/1000 dilution
Lane 1: Wild-type A431 cell lysate at 20 µg
Lane 2: Western blot - Human PTGS1 knockout A-431 cell lysate (ab270500)
Lane 2: PTGS1 knockout A431 cell lysate at 20 µg
Lane 2: Western blot - Human PTGS1 knockout A-431 cell line (ab270477)
Lane 3: C2C12 cell lysate at 20 µg
Lane 4: MOLT-4 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 70 kDa
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