Human PTN knockout U-87 MG cell line
- Advanced Validation
- What is this?
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(0 Publication)
- WB
Lab
Western blot - Human PTN knockout U-87 MG cell line (AB288713)
ab79411 was shown to react with PTN in wild-type U87mg cells in Western blot with loss of signal observed in PTN knockout cell line ab288713. Wild-type U87mg and PTN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab79411 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes:
Western blot - Anti-PTN antibody [EPR3041] (<a href='/en-us/products/primary-antibodies/ptn-antibody-epr3041-ab79411'>ab79411</a>) at 1/500 dilution
Lanes 1 - 2:
Western blot - Human PTN knockout U-87 MG cell line (ab288713)
Lane 1:
Wild-type U87mg lysate at 20 µg
Lane 2:
PTN Knockout U87mg lysate at 20 µg
Observed band size: 19 kDa
false
- WB
Lab
Western blot - Human PTN knockout U-87 MG cell line (AB288713)
ab133517 was shown to react with PTN in wild-type U87mg cells in Western blot with loss of signal observed in PTN knockout cell line ab288713. Wild-type U87mg and PTN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab133517 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes:
Western blot - Anti-PTN antibody [EP3040(2)] (<a href='/en-us/products/primary-antibodies/ptn-antibody-ep30402-ab133517'>ab133517</a>) at 1/500 dilution
Lane 1:
Wild-type U-87 MG lysate at 25 µg
Lane 2:
PTN knock-out U87mg lysate at 25 µg
false
- Sanger seq
Supplier Data
Sanger Sequencing - Human PTN knockout U-87 MG cell line (AB288713)
Functional Homozygote. 38 bp and 37 bp deletions
Reactivity data
Product details
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
EMEM + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Pleiotrophin functions as a potent mitogen promoting cell growth and angiogenesis. It does not operate as part of a larger protein complex but acts independently to influence cellular behavior. Its interaction with receptor protein tyrosine phosphatase (RPTP) modulates the tyrosine phosphorylation state of other proteins affecting downstream signaling. This interaction facilitates communication between cells and their environments influencing development and tissue maintenance.
Pathways
This protein significantly impacts the PI3K/Akt and MAPK/ERK pathways key regulators of cell survival and proliferation. PTN often collaborates with proteins like Midkine (MK) due to their functional similarities and overlapping receptor targets. These pathways help regulate cellular responses to environmental cues contributing to processes such as tissue repair and regeneration.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Complementary Products
Primary Antibodies
AB79411
KO Validated|RabMAb|Recombinant|20ul selling size
![Immunoprecipitation - Anti-PTN antibody [EPR3041] (AB79411)](https://content.abcam.com/products/images/ptn-antibody-epr3041-ab79411--immunoprecipitation-img429983.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com