Human PTPN2 knockout Jurkat cell line
- Advanced Validation
- What is this?
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PTPN2 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9; X = 10 bp deletion, 1 bp deletion, 2 bp insertion; Frameshift: 100%. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
PTN2_HUMAN, PTPN 2, PTPT, Protein tyrosine phosphatase non receptor type 2, T-cell protein-tyrosine phosphatase, TCELLPTP, Tyrosine-protein phosphatase non-receptor type 2
- NGS
Supplier Data
Next Generation Sequencing - Human PTPN2 knockout Jurkat cell line (AB274899)
Knockout achieved by CRISPR/Cas9; X = 10 bp deletion, 1 bp deletion, 2 bp insertion; Frameshift : 100%
- NGS
Supplier Data
Next Generation Sequencing - Human PTPN2 knockout Jurkat cell line (AB274899)
4 bp insertion after His29 (allele 1), 10 bp deletion after Glu27 (allele 2), 1 bp deletion after His29 (allele 3), and 2 bp insertion after His29 (allele 4) of the WT protein
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x105 cells/mL is recommended.
- Do not allow cell density to exceed 3x106 cells/mL.
Culture medium
RPMI + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TCPTP plays a role in the control of cell growth differentiation and immune response. It functions independently not as part of a larger complex. This enzyme acts as a negative regulator of signaling pathways by dephosphorylating proteins that drive these cellular processes. Its activity ensures a balance in cellular signaling preventing overactivation which could lead to uncontrolled cell proliferation or inappropriate immune responses.
Pathways
TCPTP holds significant function in the JAK-STAT and insulin signaling pathways. Within the JAK-STAT pathway TCPTP dephosphorylates members such as JAK1 and JAK3 helping modulate cytokine responses and immune regulation. In the insulin signaling pathway it influences the dephosphorylation of the insulin receptor thereby impacting insulin sensitivity. Such interactions demonstrate its involvement in key cellular processes necessary for immune function and metabolic regulation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Target data
Complementary Products
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com