PTPN2 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9; X = 10 bp deletion, 1 bp deletion, 2 bp insertion; Frameshift: 100%.
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Key facts
- Cell type
- Jurkat
- Species or organism
- Human
- Tissue
- Blood
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9; X = 10 bp deletion, 1 bp deletion, 2 bp insertion; Frameshift: 100%
Alternative names
PTN2_HUMAN, PTPN 2, PTPT, Protein tyrosine phosphatase non receptor type 2, T-cell protein-tyrosine phosphatase, TCELLPTP, Tyrosine-protein phosphatase non-receptor type 2
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PTPN2 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9; X = 10 bp deletion, 1 bp deletion, 2 bp insertion; Frameshift: 100%.
Key facts
- Cell type
- Jurkat
- Species or organism
- Human
- Tissue
- Blood
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9; X = 10 bp deletion, 1 bp deletion, 2 bp insertion; Frameshift: 100%
- Disease
- Non-Hodgkin Lymphoma
- Concentration
Properties
- Gene name
- PTPN2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Suspension
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x105 cells/mL is recommended.
- Do not allow cell density to exceed 3x106 cells/mL.
- Culture medium
- RPMI + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TCPTP or T-cell protein tyrosine phosphatase is an enzyme that functions mechanically by removing phosphate groups from tyrosine residues on its substrate proteins. This process helps regulate various signaling pathways within cells. Also known as PTPN2 TCPTP is a protein with a mass of approximately 48 kDa. Its expression occurs in many tissues with notable levels in hematopoietic cells and within the endoplasmic reticulum.
- Biological function summary
TCPTP plays a role in the control of cell growth differentiation and immune response. It functions independently not as part of a larger complex. This enzyme acts as a negative regulator of signaling pathways by dephosphorylating proteins that drive these cellular processes. Its activity ensures a balance in cellular signaling preventing overactivation which could lead to uncontrolled cell proliferation or inappropriate immune responses.
- Pathways
TCPTP holds significant function in the JAK-STAT and insulin signaling pathways. Within the JAK-STAT pathway TCPTP dephosphorylates members such as JAK1 and JAK3 helping modulate cytokine responses and immune regulation. In the insulin signaling pathway it influences the dephosphorylation of the insulin receptor thereby impacting insulin sensitivity. Such interactions demonstrate its involvement in key cellular processes necessary for immune function and metabolic regulation.
- Associated diseases and disorders
TCPTP has associations with autoimmune diseases such as Type 1 Diabetes and Crohn’s Disease. Reduced activity or altered expression of TCPTP has been observed in these conditions suggesting its regulatory role in immune tolerance. Furthermore its connection with proteins like the cytokine IL-6 in autoimmune responses implicates TCPTP in the disease mechanisms where disrupted signaling can contribute to chronic inflammation and immune system dysfunction.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Next Generation Sequencing - Human PTPN2 knockout Jurkat cell line (ab274899)
Knockout achieved by CRISPR/Cas9; X = 10 bp deletion, 1 bp deletion, 2 bp insertion; Frameshift: 100%
Next Generation Sequencing - Human PTPN2 knockout Jurkat cell line (ab274899)
4 bp insertion after His29 (allele 1), 10 bp deletion after Glu27 (allele 2), 1 bp deletion after His29 (allele 3), and 2 bp insertion after His29 (allele 4) of the WT protein
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