PYHIN1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 5 and 2 bp insertion in exon 5 and 8 bp insertion in exon 5.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 5 and 2 bp insertion in exon 5 and 8 bp insertion in exon 5
Alternative names
IFIX_HUMAN, Interferon-inducible protein X, Pyhin1, Pyrin and HIN domain-containing protein 1
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PYHIN1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 5 and 2 bp insertion in exon 5 and 8 bp insertion in exon 5.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 5 and 2 bp insertion in exon 5 and 8 bp insertion in exon 5
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- PYHIN1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
- Culture medium
- F-12K + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PYHIN1 also known as p205 or IFI16-like protein is a member of the PYHIN protein family with a mass of approximately 87 kDa. It plays a role in the innate immune response. Mechanistically PYHIN1 functions as a DNA sensor in the cytosol and nucleus detecting foreign DNA and initiating signaling cascades leading to immune activation. This protein is mainly expressed in hematopoietic cells including blood mononuclear cells and various immune tissues.
- Biological function summary
PYHIN1 contributes to immune system regulation and antiviral defense through nucleic acid sensing. It recognizes double-stranded DNA in the cytosol and interacts with other proteins to trigger the production of type I interferons and pro-inflammatory cytokines. PYHIN1 is not part of a large complex but interacts with other key proteins in the signaling pathway enhancing the immune response to viral infections and other pathogens.
- Pathways
PYHIN1 is important in signaling pathways that activate the immune system such as the STING pathway and interferon regulatory factor pathways. PYHIN1 interacts with the cGAS-STING pathway detecting cytoplasmic DNA and facilitating signal transduction for innate immune activation. It is related to proteins like STING and IRF3 which are critical for transmitting signals leading to the production of interferons and other immune molecules.
- Associated diseases and disorders
PYHIN1 is connected to inflammatory diseases and autoimmune disorders. This protein contributes to the body’s defense against viral infections which can become dysregulated and lead to pathologies like lupus. PYHIN1’s interaction with proteins such as STING and IFI16 can influence autoimmune responses and inflammation across different contexts. Understanding the function and regulation of PYHIN1 is essential in exploring therapeutic approaches for these conditions.
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3 product images
Sanger Sequencing - Human PYHIN1 knockout A549 cell line (ab267007)
Allele-2: 2 bp insertion in exon 5.
Sanger Sequencing - Human PYHIN1 knockout A549 cell line (ab267007)
Allele-3: 8 bp insertion in exon 5.
Sanger Sequencing - Human PYHIN1 knockout A549 cell line (ab267007)
Allele-1: 2 bp deletion in exon5
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