QTRT1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 1 and 46 bp deletion in exon 1.
Images
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Form
Liquid
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 1 and 46 bp deletion in exon 1
Alternative names
FP3235, Guanine insertion enzyme, Queuine tRNA ribosyltransferase 1, Queuine tRNA-ribosyltransferase, TGT, 43 KD subunit, TGT, catalytic subunit, TGT_HUMAN, TGUT, qtrt1, tRNA-guanine transglycosylase
Recommended products
QTRT1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 1 and 46 bp deletion in exon 1.
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Form
Liquid
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 1 and 46 bp deletion in exon 1
- Disease
Adenocarcinoma
- Concentration
Properties
- Gene name
QTRT1
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Sanger Sequencing
Quality control
- STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
EU: 2 US: 2
- Adherent/suspension
Adherent
- Gender
Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-196°C
- Appropriate long-term storage conditions
-196°C
Notes
Recommended control: Human wild-type HeLa cell line (Human wild-type HeLa cell line ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The TGT protein also known as tRNA-guanine transglycosylase functions mechanically by modifying transfer RNA (tRNA) molecules through the exchange of guanine at specific tRNA positions with preQ1 precursors. This enzymatic activity is essential in tRNA maturation. The enzyme has a molecular mass of about 43 kDa. TGT is expressed mainly in the cytoplasm of eukaryotic cells and shows a high level of conservation across different species.
- Biological function summary
The TGT catalyzed guanine exchange on tRNA enhances the correct folding of tRNA and supports the accuracy of protein translation. The protein is a part of an RNA-modifying enzyme family and does not typically form complexes but acts independently on its tRNA substrates. Its activity ensures proper tRNA structure influencing protein synthesis efficiency and overall cellular protein homeostasis.
- Pathways
TGT plays an important role in the tRNA modification pathway which is important for post-transcriptional RNA processing. It connects with pathways involved in protein synthesis and RNA stability. TGT's function is integrally linked with enzymes like tRNA synthetases which are responsible for charging tRNAs with their corresponding amino acids establishing a pivotal link in the genetic code translation process.
- Associated diseases and disorders
Alterations in TGT function or expression impact specific disorders such as mitochondrial myopathy which results from defects in tRNA modification leading to flawed mitochondrial protein synthesis. TGT also has connections to cancer biology where aberrant tRNA modifications by TGT might influence tumoral cell growth and response to stress. In these contexts the interactions between TGT and other proteins involved in RNA metabolism such as Dicer point to a broader influence on cellular regulatory mechanisms linked to disease states.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Sanger Sequencing - Human QTRT1 knockout HeLa cell line (ab265454)
Allele-2: 17 bp deletion in exon 1.
Sanger Sequencing - Human QTRT1 knockout HeLa cell line (ab265454)
Allele-1: 46 bp deletion in exon 1.
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