Human RAB9A (Rab9) knockout HeLa cell line
- Advanced Validation
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RAB9A KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and Insertion of the selection cassette in exon 3.
View Alternative Names
2410064E05Rik, AI195561, DmRab9, RAB9 member RAS oncogene family, RAB9A member RAS oncogene family, RAB9A_HUMAN, RAS ASSOCIATED PROTEIN RAB9, Rab 9, Ras-related protein Rab-9A, Sid6061p, Sid99
- WB
Lab
Western blot - Human RAB9A (Rab9) knockout HeLa cell line (AB265693)
Lanes 1-4 : Merged signal (red and green). Green - ab2810 observed at 25 kDa. Red - loading control ab181602 observed at 36 kDa.
ab2810 Anti-Rab9 antibody [Mab9] was shown to specifically react with Rab9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265693 (knockout cell lysate ab257625) was used. Wild-type and Rab9 knockout samples were subjected to SDS-PAGE. ab2810 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively.Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Rab9 antibody [Mab9] - Late Endosome Marker (<a href='/en-us/products/primary-antibodies/rab9-antibody-mab9-late-endosome-marker-ab2810'>ab2810</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
RAB9A knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human RAB9A (Rab9) knockout HeLa cell line (ab265693)
Lane 3:
MCF7 cell lysate at 20 µg
Lane 4:
MDA-MB-231 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa
false
- WB
Lab
Western blot - Human RAB9A (Rab9) knockout HeLa cell line (AB265693)
Lanes 1-4 : Merged signal (red and green). Green - ab179815 observed at 25 kDa. Red - loading control ab8245 observed at 36 kDa.
ab179815 Anti-Rab9 antibody [EPR13272] was shown to specifically react with Rab9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265693 (knockout cell lysate ab257625) was used. Wild-type and Rab9 knockout samples were subjected to SDS-PAGE. ab179815 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively.Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (<a href='/en-us/products/primary-antibodies/rab9-antibody-epr13272-late-endosome-marker-ab179815'>ab179815</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
RAB9A knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human RAB9A (Rab9) knockout HeLa cell line (ab265693)
Lane 3:
MCF7 cell lysate at 20 µg
Lane 4:
MDA-MB-231 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human RAB9A (Rab9) knockout HeLa cell line (AB265693)
Allele-1 : 1 bp insertion in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human RAB9A (Rab9) knockout HeLa cell line (AB265693)
Allele-2 : Insertion of the selection cassette in exon 3.
Reactivity data
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Rab9 coordinates several intracellular trafficking processes aiding in the regulation of membrane identity. It associates with other effectors such as tail-interacting protein (TIP47) to form complexes on the endosomal surface. These complexes help in vesicular transport ensuring efficient recycling of receptors. Rab9 impacts endosome maturation and transport making it an essential endosome marker.
Pathways
Rab9 influences and is involved in the regulation of the endocytic and secretory pathways. It interacts with proteins like Rab7 another GTPase to manage late endosome and lysosome transport. These interactions underline Rab9's functionalities in intracellular trafficking and recycling processes relying on cooperative interactions with other Rabs and their effectors.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Cell Lines & Lysates
AB255371
Human CAV1 (Caveolin-1) knockout HeLa cell line
Advanced Validation
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- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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