RAET1E KO cell line available to order. Free of charge wild type control provided.
MCF7
Human
Breast
Liquid
LETAL, Lymphocyte effector toxicity activation ligand, MGC125308, MGC125309, N2DL-4, N2DL4_HUMAN, NKG2D ligand 4, NKG2D ligand 4 precursor, NKG2DL4, RAE-1-like transcript 4, RAET1E2, Retinoic acid early transcript 1E, bA350J20.7
RAET1E KO cell line available to order. Free of charge wild type control provided.
MCF7
Human
Breast
Liquid
Adenocarcinoma
RAET1E
Knockout
CRISPR technology
EU: 1 US: 1
Adherent
Female
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
MEM + 10% FBS + 0.01 mg/ml bovine insulin
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Dry Ice
-196°C
-196°C
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type MCF7 cell line (Human wild-type MCF7 cell line ab288560). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
This supplementary information is collated from multiple sources and compiled automatically.
RAET1E also known as UL16-binding protein 4 (ULBP4) is a member of the MHC class I-related molecules. It has a molecular mass of approximately 31 kDa. RAET1E is part of the family of proteins that acts as a ligand for the NKG2D receptor found on the surface of natural killer (NK) cells. In terms of expression RAET1E is present on the surface of various cell types especially in stressed transformed or virus-infected cells where it signals cellular distress to immune cells.
RAET1E plays a role in immune surveillance. It is involved in the activation of NK cells when it binds to the NKG2D receptor. This interaction is part of the body's defense mechanism against transformed or infected cells. RAET1E operates in isolation rather than as part of a larger protein complex which allows it to independently initiate the immune response when detecting harmful cells.
RAET1E functions within the innate immune response pathway. It works closely with the NKG2D signaling pathway which is important for the activation of cytotoxic activities in immune cells. RAET1E through its binding to NKG2D facilitates the destruction of malignant cells. This connection places RAET1E alongside other similar NKG2D ligands such as MIC and other ULBP family proteins which contribute to the activation of NK and T cells.
RAET1E is linked to various cancer types and infectious diseases. Its expression on the surface of tumor cells can signal NK cells to target and destroy these potentially harmful cells. In certain cancers the loss or reduction of RAET1E expression may allow tumor cells to escape immune detection. In viral infections the upregulation of RAET1E on infected cells assists in the clearance of viruses by NK cells. Both in cancer and viral infections proteins like RAET1E and NKG2D play an important part in the immune system's defensive strategies.
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