RBM4 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2
Alternative names
Lark homolog, RBM4_HUMAN, RNA binding motif 4, RNA binding motif 4a, RNA-binding motif protein 4, RNA-binding motif protein 4a, RNA-binding protein 4, dkfzp547k0918, hLark, lark, lark homologue, mgc75138, rbm4 lark
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RBM4 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2
- Concentration
Properties
- Gene name
- RBM4
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RNA Binding Motif Protein 4 (RBM4) also known as LARK1 is a RNA-binding protein with a molecular mass of approximately 37 kDa. This protein plays a role in post-transcriptional regulation by binding to RNA and influencing alternative splicing. RBM4 mainly expresses in the cytoplasm of various tissues including muscle and neuronal tissues and is involved in the regulation of mRNA synthesis and turnover. Its function relies on the presence of RNA recognition motifs that facilitate its binding to RNA targets.
- Biological function summary
RNA Binding Motif Protein 4 impacts cellular processes through its ability to modulate alternative splicing and mRNA translation. It has been shown to form complexes with other RNA-binding proteins and splicing factors enhancing its regulatory capabilities. Through these interactions RBM4 can influence the expression of a wide array of genes involved in cellular development and differentiation adapting protein synthesis to meet cellular and environmental cues.
- Pathways
RBM4's function contributes significantly to the regulation of insulin signaling and muscle differentiation pathways. By interacting with other proteins such as SRSF1 and SRPK1 RBM4 aids in modulating the gene splicing required for effective insulin response and muscle cell formation. These interactions ensure that the pathways remain responsive to internal cellular signals and external metabolic demands coordinating essential physiological processes.
- Associated diseases and disorders
RNA Binding Motif Protein 4 has been linked to the development of metabolic disorders and certain types of cancer. Aberrant expression or mutations in RBM4 can disrupt normal insulin signaling which may result in type 2 diabetes. In cancer its altered activity can affect cell cycle regulation and apoptosis through connections with other proteins like p53 contributing to tumorigenesis and cancer progression. Understanding the role of RBM4 in these conditions highlights its therapeutic potential as a target for intervention.
Product promise
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1 product image
Sanger Sequencing - Human RBM4 knockout HEK-293T cell line (ab266639)
Homozygous: 19 bp deletion in exon 2
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