REPIN1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 1 and 2 bp deletion in exon 1.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 1 and 2 bp deletion in exon 1
Alternative names
60 kDa origin specific DNA binding protein, 60 kDa replication initiation region protein, ATT binding protein, DHFR oribeta binding protein RIP60, H_DJ0584D14.12, REPIN1, RIP60, Replication initiation region protein (60kD), Replication initiator 1, ZNF464, Zfp464, Zinc finger protein 464 (RIP60), Zinc finger proten AP4
Recommended products
REPIN1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 1 and 2 bp deletion in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 1 and 2 bp deletion in exon 1
- Concentration
Properties
- Gene name
- REPIN1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AP4 also known as Adaptor Protein Complex 4 plays an important role in the transport of molecules within the cell. It operates as part of the family of adaptor protein complexes involved in trafficking cargo proteins between membranes. AP4 is a medium-sized protein complex with a mass ranging approximately from 300 to 500 kDa. It predominantly expresses itself in neuronal tissues where it facilitates intracellular trafficking and protein sorting essential for cellular functions.
- Biological function summary
AP4 is an essential piece of the vesicular transport machinery. It interacts with various cargo proteins sorting them into distinct vesicles for different cellular destinations. AP4 functions as part of a complex that includes other adaptins and accessory proteins each contributing specialized roles for accurate protein sorting. These interactions play significant roles in maintaining cellular homeostasis and normal neuronal function.
- Pathways
AP4 is intimately involved in the endosomal-lysosomal pathway and clathrin-mediated endocytosis. These pathways are important for proper cellular protein recycling and degradation processes. AP4 also associates with other proteins like AP1 and AP2 which overlap in the sorting at different cellular locations. These connections make AP4 an essential component of the broader protein transport network within cells.
- Associated diseases and disorders
Research links AP4 to certain neurodegenerative conditions and hereditary spastic paraplegia. Mutations in AP4-related genes can disrupt normal protein trafficking affecting neuronal health potentially through a combined action with the SNX3 protein. AP4’s dysfunction in these contexts illustrates its importance in maintaining neural connectivity and function highlighting its potential as a therapeutic target in related disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sanger Sequencing - Human REPIN1 (AP4) knockout HEK-293T cell line (ab266363)
Allele-2: 2 bp deletion in exon 1.
Sanger Sequencing - Human REPIN1 (AP4) knockout HEK-293T cell line (ab266363)
Allele-1: 13 bp deletion in exon 1
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