Human RET knockout MCF7 cell line
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RET KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided.
View Alternative Names
C ret, CDHF 12, CDHR16, Cadherin family member 12, Cadherin related family member 16, ELKS Fusion gene, HSCR 1, MEN2A, MEN2B, MTC 1, Multiple endocrine neoplasia and medullary thyroid carcinoma 1, Oncogene RET, PTC, Proto-oncogene c-Ret, Proto-oncogene tyrosine-protein kinase receptor ret, RET ELE1, RET transforming sequence, RET51, RET9, RET_HUMAN, Ret Proto oncogene, hydroxyaryl-protein kinase, tyrosine-protein kinase receptor ret
- NGS
Lab
Next Generation Sequencing - Human RET knockout MCF7 cell line (AB286279)
104 bp deletion (allele 1) and 37 bp deletion in exon 4, CCDS7200.1
Reactivity data
Product details
Recommended control: Human wild-type MCF7 cell line (ab288560). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- Slow to trypsinise.
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 5-7x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
MEM + 10% FBS + 0.01 mg/ml bovine insulin
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Ret protein facilitates several critical processes within the body. It functions as part of a complex with co-receptors called GFRα (GDNF family receptor alpha) which are required for binding ligands such as GDNF (glial cell line-derived neurotrophic factor). This binding activates downstream signaling pathways that influence cell growth differentiation and survival particularly within the nervous system. The proper functioning of Ret is essential for the development and maintenance of these cellular environments.
Pathways
Ret is an important component of the MAPK/ERK and PI3K/AKT signaling pathways. These pathways mediate various cellular responses including cell proliferation survival and apoptosis. The interactions of Ret with other proteins like SHC and GRB2 within these pathways create ripple effects that impact larger cellular networks. Through these pathways Ret connects with multiple proteins to modulate essential biological processes.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
![Western blot - Anti-Ret antibody [EPR2871] (AB134100)](https://content.abcam.com/products/images/ret-antibody-epr2871-ab134100--western-blot-img438708.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com