RING1 KO cell line available to order. Free of charge wild type control provided.
PC-3
Human
Prostate
Liquid
E3 ubiquitin-protein ligase RING1, Polycomb complex protein RING1, RING finger protein 1, RING1A, RING1_HUMAN, Really interesting new gene 1 protein, Rnf1, Transcription repressor Ring1A
RING1 KO cell line available to order. Free of charge wild type control provided.
PC-3
Human
Prostate
Liquid
Adenocarcinoma
RING1
Knockout
CRISPR technology
EU: 1 US: 1
Adherent
Male
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
F-12K + 10% FBS
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Dry Ice
-196°C
-196°C
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type PC-3 cell line (Human wild-type PC-3 cell line ab290718). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
This supplementary information is collated from multiple sources and compiled automatically.
RING1 also known as Ring Finger Protein 1 or RNF1 is an E3 ubiquitin-protein ligase with a molecular mass of approximately 42 kDa. It is part of the polycomb group proteins known for regulating gene expression. RING1 is expressed ubiquitously in various tissues where it functions as an important regulator of protein degradation by tagging substrates with ubiquitin for proteasomal degradation. This activity plays a critical role in maintaining cellular homeostasis by controlling the abundance of regulatory proteins.
Along with modulating protein turnover RING1 participates as a fundamental component of the Polycomb Repressive Complex 1 (PRC1). This complex is imperative in chromatin remodeling and transcriptional repression influencing epigenetic gene silencing. RING1 works in tandem with other proteins such as BMI1 facilitating histone ubiquitination and altering chromatin structure which impacts gene expression across different cell types and developmental stages.
The ubiquitin-proteasome system and the epigenetic regulation pathway involve RING1. Within the ubiquitin-proteasome system RING1 interacts with other E3 ligases and ubiquitin-conjugating enzymes governing the fate of its protein substrates. In the epigenetic regulation pathway RING1's role in PRC1 places it alongside proteins like EZH2 contributing to the repression of genes involved in differentiation and development thereby ensuring proper cell identity maintenance.
Mutations or misregulation of RING1 have links to several conditions including cancers and neurodegenerative disorders. In cancer the disruption of normal RING1 functions can lead to unregulated cell proliferation due to improper gene silencing. For neurodegenerative disorders associations with proteins such as ubiquitin and its regulatory roles may contribute to disease pathogenesis by enabling the accumulation of misfolded proteins further highlighting its significance in cellular health and disease.
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